BD Pharmingen™ PE Rat Anti-Mouse Treml4
Clone 16E5 (RUO)
Flow cytometric analysis of TREML4 expression on Mouse splenic leucocytes. C57BL/6 Mouse spleen cells were treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with BD Horizon™ BUV395 Rat Anti-Mouse CD8a (Cat No. 563786) and APC Hamster Anti-Mouse CD11c (Cat No. 550261) antibodies and with either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; Dotted Line Histogram) or PE Rat Anti-Mouse TREML4 antibody (Cat. No. 568948/568949; Solid Line Histogram) at 0.5 μg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing the expression of TREML4 (or Ig Isotype control staining) was derived from gated events with the forward and side light- scatter characteristics of viable (DAPI-negative) CD8a and CD11c positive leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of TREML4 expression on Mouse splenic leucocytes. C57BL/6 Mouse spleen cells were treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with BD Horizon™ BUV395 Rat Anti-Mouse CD8a (Cat No. 563786) and APC Hamster Anti-Mouse CD11c (Cat No. 550261) antibodies and with either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; Dotted Line Histogram) or PE Rat Anti-Mouse TREML4 antibody (Cat. No. 568948/568949; Solid Line Histogram) at 0.5 μg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing the expression of TREML4 (or Ig Isotype control staining) was derived from gated events with the forward and side light- scatter characteristics of viable (DAPI-negative) CD8a and CD11c positive leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Companion Products
The 16E5 monoclonal antibody specifically recognizes Trem-like 4 (Treml4) which is also known as Trem-like transcript 4 protein (TLT-4 or TLT4). Treml4 is a type I transmembrane glycoprotein protein that is encoded by Treml4 (Treml4 triggering receptor expressed on myeloid cells-like 4). Treml4 is comprised of a single IgV-like domain in its extracellular region that is followed by a transmembrane segment and a short cytoplasmic tail. Treml4 is expressed on splenic macrophages and dendritic cells (DC), particularly CD8+ dendritic cells. Treml4 can associate with DAP12 and regulate immune responses. It reportedly binds to B7-H3 and to apoptotic or necrotic cells.
Development References (3)
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Hemmi H, Idoyaga J, Suda K, et al. A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.. J Immunol. 2009; 182(3):1278-86. (Immunogen: Flow cytometry, Western blot). View Reference
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Hemmi H, Zaidi N, Wang B, et al. Treml4, an Ig superfamily member, mediates presentation of several antigens to T cells in vivo, including protective immunity to HER2 protein.. J Immunol. 2012; 188(3):1147-55. (Clone-specific: Flow cytometry). View Reference
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Pek RH, Yuan X, Rietzschel N, et al. Hemozoin produced by mammals confers heme tolerance.. Elife. 2019; 8:e49503. (Clone-specific: Flow cytometry). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.