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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
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13R4.rMAb is a recombinant monoclonal antibody that specifically recognizes Escherichia coli β-galactosidase, which is also known as beta-galactosidase or beta-gal. β-galactosidase is a cytoplasmatic metalloenzyme encoded by the lacZ gene in the lac operon of E. coli. β-galactosidase catalyzes the hydrolysis of lactose to its constituent monosaccharides glucose and galactose. E. coli β-galactosidase is often used as a tag for recombinant mammalian proteins because it is not expressed by mammalian cells. This permits easy detection and purification of β-galactosidase-tagged recombinant proteins expressed by genetically engineered mammalian cells.
Development References (5)
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Juers DH, Matthews BW, Huber RE. LacZ β-galactosidase: structure and function of an enzyme of historical and molecular biological importance.. Protein Sci. 2012; 21(12):1792-807. (Biology). View Reference
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Laden JC, Philibert P, Torreilles F, Pugnière M, Martineau P. Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm.. Res Microbiol. 2002; 153(7):469-74. (Biology). View Reference
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Martineau P, Jones P, Winter G. Expression of an antibody fragment at high levels in the bacterial cytoplasm.. J Mol Biol. 1998; 280(1):117-27. (Immunogen: ELISA, Western blot). View Reference
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Philibert P, Martineau P. Directed evolution of single-chain Fv for cytoplasmic expression using the beta-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation.. Microb Cell Fact. 2004; 3(1):16. (Clone-specific: Functional assay). View Reference
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Sibler AP, Courtête J, Muller CD, Zeder-Lutz G, Weiss E. Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells.. FEBS J. 2005; 272(11):2878-91. (Clone-specific: Fluorescence activated cell sorting, Intracellular Staining/Flow Cytometry, Western blot). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.