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Two-color flow cytometric analysis of TACTILE (CD96) expression on human peripheral blood lymphocytes. The flow cytometric data from the same stained cells was reanalyzed to generate the bivariate pseudocolor density plot showing the correlated expression of TACTILE (CD96) [or Ig Isotype control staining] versus CD3 for gated events with the forward and side light-scatter characteristics of viable human lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Multiparameter flow cytometric analysis of TACTILE (CD96) expression on human peripheral blood leucocytes. Human whole blood was stained with APC Mouse Anti-Human CD3 antibody (Cat No. 561811) and either PE Mouse IgG1, κ Isotype Control (Cat No. 554680; Left Plot) or PE Mouse Anti-Human TACTILE (CD96) antibody (Cat No. 568271/568272; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat No. 555899). The bivariate pseudocolor density plot showing the correlated expression of TACTILE (CD96) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable human leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
BD Pharmingen™ PE Mouse Anti-Human TACTILE (CD96)
BD Pharmingen™ PE Mouse Anti-Human TACTILE (CD96)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
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Companion Products
The NK92.39.rMAb monoclonal antibody specifically recognizes human CD96 which is also known as TACTILE (T cell activation increased late expression). CD96 is a type I membrane glycoprotein comprised of three N-terminal Ig-like extracellular domains followed by one mucin-like domain, a transmembrane region, and a cytoplasmic tail with one basic/proline rich motif, a single ITIM motif, and an YXXM motif which is also present in ICOS and CD28. CD96 is expressed at low levels on resting natural killer (NK) cells and T cells and at high levels on activated NK and T cells. CD96 is also expressed at low levels on some B cells and on some T-cell leukemia and acute myeloid leukemia cells. CD96 plays a role in the adhesive interactions of activated NK and T cells with target cells during immune responses. CD96 binds to the poliovirus receptor (CD155) that is highly expressed on some tumor cells. CD155-mediated ligation of CD96 can activate NK cell-mediated cytotoxicity.
Development References (3)
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El-Sherbiny YM, Meade JL, Holmes TD, et al. The requirement for DNAM-1, NKG2D, and NKp46 in the natural killer cell-mediated killing of myeloma cells.. Cancer Res. 2007; 67(18):8444-9. (Clone-specific: Flow cytometry). View Reference
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Fuchs A, Cella M, Giurisato E, Shaw AS, Colonna M. Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155).. J Immunol. 2004; 172(7):3994-8. (Immunogen: Blocking, Flow cytometry). View Reference
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Toutirais O, Cabillic F, Le Friec G, et al. DNAX accessory molecule-1 (CD226) promotes human hepatocellular carcinoma cell lysis by Vgamma9Vdelta2 T cells.. Eur J Immunol. 2009; 39(5):1361-8. (Clone-specific: Flow cytometry, Functional assay). View Reference
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