Skip to main content Skip to navigation
PE Mouse Anti-Human STING
PE Mouse Anti-Human STING
Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) followed by permeabilization with BD Perm/Wash™ Buffer (Cat. No. 554723). Cells were then stained with BD Horizon™ BV421 Mouse Anti-Human CD20 antibody (Cat. No. 563346) and either PE IgG1, κ Isotype Control (Cat. No. 554680, Top Panels) or PE Mouse Anti-Human STING antibody (Cat. No. 564965; Bottom Panels).      Left Panels - Contour plots showing the correlated expression of STING (or Ig Isotype control staining) versus Side Light Scatter (SSC) were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes.       Right Panels - Contour plots showing the correlated expression of STING (or Ig Isotype control staining) versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.      Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) followed by permeabilization with BD Perm/Wash™ Buffer (Cat. No. 554723). Cells were then stained with BD Horizon™ BV421 Mouse Anti-Human CD20 antibody (Cat. No. 563346) and either PE IgG1, κ Isotype Control (Cat. No. 554680, Top Panels) or PE Mouse Anti-Human STING antibody (Cat. No. 564965; Bottom Panels).      Left Panels - Contour plots showing the correlated expression of STING (or Ig Isotype control staining) versus Side Light Scatter (SSC) were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes.       Right Panels - Contour plots showing the correlated expression of STING (or Ig Isotype control staining) versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.      Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
Down Arrow Up Arrow


BD Pharmingen™
hSTING; MITA; hMITA; ERIS; MPYS; NET23; TM173; TMEM173
Human (QC Testing)
Mouse IgG1, κ
Human STING Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
AB_2739026
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
564965 Rev. 1
Antibody Details
Down Arrow Up Arrow
T3-680

The T3-680 monoclonal antibody specifically recognizes human STING (Stimulator of interferon genes protein) which is also known as Endoplasmic reticulum interferon stimulator (ERIS) and Mediator of IRF3 activation (MITA). STING is encoded by TMEM173 (Transmembrane protein 173). STING is a multi-pass transmembrane adaptor protein which is widely expressed in the endoplasmic reticulum of cells. STING acts as a sensor of cytosolic double-stranded DNA derived from bacteria and viruses and promotes the production of type I interferons, IFN-α and IFN-β. Following activation, STING translocates with TBK1 kinase to perinuclear endosomes. The TBK1 kinase phosphorylates and activates interferon regulatory factors, such as IRF3, and NF-κB, which leads to the induction of type I interferon and other immune response genes that play roles in innate and adaptive immunity.

564965 Rev. 1
Format Details
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
564965 Rev.1
Citations & References
Down Arrow Up Arrow
View product citations for antibody "564965" on CiteAb

Development References (5)

  1. Ablasser A, Goldeck M, Cavlar T, et al. cGAS produces a 2'-5'-linked cyclic dinucleotide second messenger that activates STING. Nature. 2013; 498(7454):380-384. (Biology). View Reference
  2. Ishikawa H, Barber GN. STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling. Nature. 2008; 455(7213):674-678. (Biology). View Reference
  3. Sun L, Wu J, Du F, Chen X, Chen ZJ. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science. 2012; 339(6121):786-791. (Biology). View Reference
  4. Unterholzner L, Keating SE, Baran M, et al. IFI16 is an innate immune sensor for intracellular DNA. Nat Immunol. 2010; 11(11):997-1004. (Biology). View Reference
  5. Zhang Z, Yuan B, Bao M, Lu N, Kim T, Liu YJ. The helicase DDX41 senses intracellular DNA mediated by the adaptor STING in dendritic cells. Nat Immunol. 2012; 12(10):959-965. (Biology). View Reference
View All (5) View Less
564965 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.