PE Mouse Anti-Human Desmoglein 2

BD Pharmingen™ PE Mouse Anti-Human Desmoglein 2

Name: PE Mouse Anti-Human Desmoglein 2
Brand: BD Pharmingen™
SKU: 568854

Clone 6D8

(RUO)

Flow cytometric analysis of Desmoglein 2 expression on Human CD34+ lymphoid cells. Human peripheral blood mononuclear cells were stained with APC Mouse Anti-Human CD34 antibody (Cat. No. 560940) and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human Desmoglein 2 antibody (Cat. No. 568854/568855; solid line histogram). The fluorescence histogram showing Desmoglein 2 expression (or Ig Isotype control staining) [Right Plot] was derived from gated CD34 positive cells [Left Plot as indicated] with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

Flow cytometric analysis of Desmoglein 2 expression on Human HeLa cells. Cells from the HeLa (Cervical adenocarcinoma, ATCC CCL-2) cell line were stained with PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or with PE Mouse Anti-Human Desmoglein 2 antibody (Cat. No. 568854/568855; solid line histogram). The fluorescence histograms showing the expression of Human Desmoglein 2 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

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Product Details

Brand: BD Pharmingen™

Alternative Name: DSG2; CDHF5; Cadherin family member 5; Desmoglein-2; desmoglein-2

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Human Full-length recomb. Desmoglein 2

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl/test

Entrez Gene ID: 1829

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

APC Mouse Anti-Human CD34 RUO

Size 25 Tests Cat No. 560940

PE Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554680

568854 Rev. 2

Antibody Details

6D8

The 6D8 monoclonal antibody specifically recognizes the extracellular region of human Desmoglein 2 which is also known as Cadherin family member 5 (CDHF5). Desmoglein 2 is a type 1 transmembrane glycoprotein encoded by DSG2 which belongs to the cadherin cell adhesion molecule superfamily. Desmoglein 2 is variably expressed by epithelial cells, cardiomyocytes, endothelial progenitors associated with neoangiogenesis, and by CD34+CD45lo hematopoietic stem and progenitor cells. Desmoglein-2 plays essential roles in cellular adhesion, migration, proliferation, apoptosis and intracellular signaling. This calcium-dependent adhesion molecule is an essential component of intercellular desmosome junctions. These adhesive desmosomal junctions serve to link adjacent cells together such as in the formation of the intestinal epithelial barrier. The desmosomes can also connect with the cytoskeleton through interactions with cytoplasmic plaque proteins and intermediate filaments. Desmoglein 2 can also serve as a receptor for adenoviruses.

568854 Rev. 2

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

568854 Rev.2

Citations & References

View product citations for antibody "568854" on CiteAb

Development References (5)

Ebert LM, Tan LY, Johan MZ, et al. A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis.. Angiogenesis. 2016; 19(4):463-86. (Clone-specific: Flow cytometry). View Reference
Ebert LM, Vandyke K, Johan MZ, et al. Desmoglein-2 expression is an independent predictor of poor prognosis patients with multiple myeloma.. Mol Oncol. 2022; 16(6):1221-1240. (Clone-specific: Flow cytometry). View Reference
Tan LY, Mintoff C, Johan MZ, et al. Desmoglein 2 promotes vasculogenic mimicry in melanoma and is associated with poor clinical outcome.. Oncotarget. 2016; 7(29):46492-46508. (Clone-specific: Flow cytometry). View Reference
Wahl JK, Sacco PA, McGranahan-Sadler TM, Sauppé LM, Wheelock MJ, Johnson KR. Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains.. J Cell Sci. 1996; 109 ( Pt 5):1143-54. (Immunogen: Immunoprecipitation, Radioimmunoassay). View Reference
Wang H, Li ZY, Liu Y, et al. Desmoglein 2 is a receptor for adenovirus serotypes 3, 7, 11 and 14.. Nat Med. 2011; 17(1):96-104. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference

View All (5)

568854 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.