BD Pharmingen™ PE Hamster Anti-Mouse CD209b (SIGN-R1)
Clone 22D1 (RUO)
Multiparameter flow cytometric analysis of CD209b (SIGN-R1) expression on BALB/c or C57BL/6 Mouse resident peritoneal cells. Resident peritoneal cells were harvested from BALB/c (Top Plots) or C57BL/6 (Bottom Plots) Mice and were washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with Alexa Fluor™ 647 Rat Anti-CD11b antibody (Cat. No. 557686) and with either PE Hamster Isotype Control (Cat. No. 550085, Left Plots) or PE (Armenian) Hamster Anti-Mouse CD209b (SIGN-R1) antibody (Cat. No. 568962/568963, Right Plots) at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD209b (SIGN-R1) [or Ig Isotype control staining] versus CD11b were derived from gated events with the forward and side-light scatter characteristics of viable (DAPI-negative) resident peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of CD209b (SIGN-R1) expression on BALB/c or C57BL/6 Mouse resident peritoneal cells. Resident peritoneal cells were harvested from BALB/c (Top Plots) or C57BL/6 (Bottom Plots) Mice and were washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with Alexa Fluor™ 647 Rat Anti-CD11b antibody (Cat. No. 557686) and with either PE Hamster Isotype Control (Cat. No. 550085, Left Plots) or PE (Armenian) Hamster Anti-Mouse CD209b (SIGN-R1) antibody (Cat. No. 568962/568963, Right Plots) at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD209b (SIGN-R1) [or Ig Isotype control staining] versus CD11b were derived from gated events with the forward and side-light scatter characteristics of viable (DAPI-negative) resident peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Companion Products
The 22D1 monoclonal antibody specifically recognizes mouse CD209 antigen-like protein B (CD209b) which is also known as Mouse SIGN-related 1 (mSIGNR1 or SIGN-R1). CD209b is a mouse homolog of human CD209 (DC-SIGN) and is also referred to as DC-SIGN-related protein 1 (DC-SIGNR1). CD209b is an ~37 kDa type II transmembrane glycoprotein that is encoded by Cd209b (CD209b antigen) which belongs to the C-type lectin family. It contains a single C-terminal C-type lectin domain and is involved in the recognition and internalization of microbial polysaccharides. CD209b is expressed on macrophages residing in the splenic marginal zone, the medullary and trabecular sinuses of lymph nodes, and peritoneal cavity. CD209b is involved in the clearance and innate immune responses to microbial molecules including polysaccharides and lipopolysaccharides (LPS) and the uptake of encapsulated microbes. It may also affect leucocyte migration through its adhesive interaction with CD54 (ICAM-2).
Development References (4)
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Camara A, Lavanant AC, Abe J, et al. CD169+ macrophages in lymph node and spleen critically depend on dual RANK and LTbetaR signaling.. Proc Natl Acad Sci U S A. 2022; 119(3):e2108540119. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
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Kang YS, Kim JY, Bruening SA, et al. The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen.. Proc Natl Acad Sci USA. 2004; 101(1):215-20. (Immunogen: Flow cytometry, Fluorescence microscopy, In vivo exacerbation, Western blot). View Reference
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Kawauchi Y, Igarashi M, Kojima N. C-type lectin receptor SIGNR1 expressed on peritoneal phagocytic cells with an immature dendritic cell-like phenotype is involved in uptake of oligomannose-coated liposomes and subsequent cell maturation.. Cell Immunol. 2014; 287(2):121-8. (Clone-specific: Flow cytometry). View Reference
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Taylor PR, Martinez-Pomares L, Stacey M, Lin HH, Brown GD, Gordon S. Macrophage receptors and immune recognition.. Annu Rev Immunol. 2005; 23:901-44. (Clone-specific: In vivo exacerbation). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.