The C3 monoclonal antibody specifically reacts with epitope S of the rat MHC class I antigen, haplotypes RT1[a] (e.g., ACI, DA, COP) and RT1[b] (e.g., BP, BUF). It does not react with RT1[c] (e.g., AUG, PVG/c), RT1 (e.g., F344, LEW), or RT1[u] (e.g., AO, LOU, WF).
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.