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BV421 Rat Anti-Mouse Mer
BV421 Rat Anti-Mouse Mer

Two-color flow cytometric analysis of Mer expression on normal mouse peritoneal cavity cells (PEC). PEC from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141 or 553142). The cells were then stained with Alexa Fluor® 647 Rat Anti-Mouse F4/80 (Cat. No. 565853 or 565854) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Plot) or BD OptiBuild™ BV421 Rat Anti-Mouse Mer (Cat. No. 747837; Right Plot) at 1 μg/test. The pseudocolor dot plots showing Mer (or Ig Isotype Control) staining versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable peritoneal cavity cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

The above is qualification data only and does not represent a specific OptiBuild™ lot.

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Two-color flow cytometric analysis of Mer expression on normal mouse peritoneal cavity cells (PEC). PEC from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141 or 553142). The cells were then stained with Alexa Fluor® 647 Rat Anti-Mouse F4/80 (Cat. No. 565853 or 565854) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Plot) or BD OptiBuild™ BV421 Rat Anti-Mouse Mer (Cat. No. 747837; Right Plot) at 1 μg/test. The pseudocolor dot plots showing Mer (or Ig Isotype Control) staining versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable peritoneal cavity cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

The above is qualification data only and does not represent a specific OptiBuild™ lot.

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Product Details
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BD OptiBuild™
Mer; Mertk; nmf12; proto-oncogene c-Mer; Eyk; Nyk; Tyro 12
Mouse (Tested in Development)
Rat IgG2a
Mouse Mer Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
AB_2872300
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  9. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
747837 Rev. 1
Antibody Details
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108928

The 108928 monoclonal antibody specifically recognizes the Mer tyrosine kinase (Mer or MerTK). Mer is a single-pass type I transmembrane glycoprotein that is encoded by Mertk (c-mer proto-oncogene tyrosine kinase) and is also known as Eyk, Nyk, Tyro 12, or Nmf12. Mer is comprised of an extracellular region with two immunoglobulin (Ig)-like domains and two fibronectin type III (FNIII) domains, a transmembrane segment and a conserved intracellular tyrosine kinase domain. Tyro-3, Axl, and Mer constitute the TAM subfamily of receptor tyrosine kinases (RTK). Mer is variably expressed by multiple cell types including monocytes, macrophages, dendritic cells, NK cells, platelets, and epithelial cells including retinal pigment epithelial cells. Mer binds to the vitamin K-dependent Growth arrest-specific protein 6 (Gas6) and to Protein S (ProS) through its extracellular Ig-like domains. Ligand binding leads to receptor dimerization, and autophosphorylation of tyrosine residues within the cytoplasmic Mer domains. This results in the activation of downstream signaling pathways that control cellular adhesion, aggregation, phagocytosis/efferocytosis, proliferation, survival, and migration. Through these activities, Mer plays major roles involved in development and in the regulation of hematopoiesis and immunity. Abnormal expression of Mer has been observed in various cancers and by certain tumor cell lines.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max near 407 nm and Em Max near 421 nm, BD Horizon BV421 can be excited by the violet laser (405 nm) and detected with a 450/50 nm filter. BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously.

747837 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
747837 Rev.1
Citations & References
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Development References (6)

  1. Caraux A, Lu Q, Fernandez N, et al. Natural killer cell differentiation driven by Tyro3 receptor tyrosine kinases.. Nat Immunol. 2006; 7(7):747-54. (Biology). View Reference
  2. Cnops J, De Trez C, Stijlemans B, et al. NK-, NKT- and CD8-Derived IFNγ Drives Myeloid Cell Activation and Erythrophagocytosis, Resulting in Trypanosomosis-Associated Acute Anemia.. PLoS Pathog. 2015; 11(6):e1004964. (Clone-specific: Flow cytometry). View Reference
  3. Gould WR, Baxi SM, Schroeder R, et al. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.. J Thromb Haemost. 2005; 3(4):733-41. (Clone-specific: Flow cytometry). View Reference
  4. Lemke G, Rothlin CV. Immunobiology of the TAM receptors. Nat Rev Immunol. 2008; 8(5):327-336. (Biology). View Reference
  5. Paolino M, Choidas A, Wallner S, et al. The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells.. Nature. 2014; 507(7493):508-12. (Clone-specific: Flow cytometry). View Reference
  6. Taichman RS, Patel LR, Bedenis R, et al. GAS6 receptor status is associated with dormancy and bone metastatic tumor formation.. PLoS ONE. 2013; 8(4):e61873. (Clone-specific: Flow cytometry). View Reference
View All (6) View Less
747837 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.