The Zt/g4 monoclonal antibody specifically binds to RON, which is also known as CD136, Macrophage-stimulating protein receptor (MSP-Receptor, MSP-R), Macrophage stimulating 1 receptor (c-met-related tyrosine kinase) (MST1R), or Protein-tyrosine kinase 8 (PTK8). RON is a type I transmembrane glycoprotein that belongs to the receptor tyrosine kinase family. It is expressed on cells as a disulfide-linked heterodimer. RON is expressed on macrophages, keratinocytes, erythroblasts and epithelial cells from the skin, lung, kidney, liver, intestine, colon, and bone marrow. RON reportedly regulates many physiological processes including the survival, migration, differentiation, and effector functions of a variety of cell types including epithelial cells, and macrophages. RON binds Macrophage stimulating protein (MSP). Overexpression of RON may contribute to the malignant transformation of epithelial cells.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.