The HLY9.1.25 monoclonal antibody specifically binds to CD229 which is also known as, Signaling lymphocyte activation molecule family member 3 (SLAMF3), Lymphocyte antigen 9 (Ly9), or T-lymphocyte surface antigen Ly-9. CD229 is a 100-120 kDa, single-pass type I membrane glycoprotein that belongs to the SLAM family within the Ig superfamily. CD229 is expressed on thymocytes, T cells, B cells, dendritic cells, and NK cells. Through homophilic binding, CD229 may play a role in adhesive interactions between T and B cells. During T cell activation, CD229 localizes to the immunological synapse.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.