The 401901 monoclonal antibody specifically recognizes Junctional adhesion molecule-like (JAML) which is also known as Adhesion molecule interacting with CXADR antigen 1 (AMICA), or Dendritic cell-specific protein CREA7-1. JAML (AMICA) is a ~65 kDa single-pass type I transmembrane glycoprotein that is encoded by JAML which belongs to the junctional adhesion molecule (JAM) family within the Ig gene superfamily. The extracellular region of this adhesion molecule contains two Ig domains followed by a transmembrane segment and a cytoplasmic domain involved in activation signaling. JAML (AMICA) is expressed on some leucocytes including granulocytes, monocytes, γδ T cells and effector memory αβ T cells. Leucocyte JAML (AMICA) forms a homodimer that can interact with CXADR (Coxsackie virus and adenovirus receptor, also known as CAR), a cell-surface membrane receptor found in the tight junctional complexes of adjacent endothelial or epithelial cells. These heterophilic interactions can function in leucocyte activation and the regulation of leucocyte migration through epithelial and endothelial tissues, eg, during inflammatory responses or wound healing.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).