CD85 molecules belong to a large immunoregulatory family and it has been clustered into different subclasses from CD85a to CD85m in the VIIth HLDA workshop. CD85j is also called as Ig-like transcript (ILT2), or leukocyte Ig-like receptor (LIR-1). Reacts with an 110 kDa membrane glycoprotein expressed on a subset of NK cells, which varies amongst individuals, and a subpopulation of T lymphocytes. Expression on T lymphocytes, NK cells may depend on the individuals tested. Function studies show that ligation of ILT2 with MHC class I including HLA-A, B, G1 and -E induces an inhibitory signal via recruitment of SHP-1 phosphatase.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).