The A12 monoclonal antibody specifically binds to CD150, a single chain type I transmembrane glycoprotein of approximately 75-95 kDa. CD150 is encoded by the SLAMF1 gene and is also known as a signaling lymphocyte activation molecule (SLAM), or IPO-3. It is expressed on T cells, B cells, thymocytes, germinal center and dendritic cells, but not on monocytes. Expression of CD150 is upregulated on T, B and dendritic cells after activation via T-cell and B-cell costimulatory molecules. It has been reported that CD150 plays a role in signal transduction.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).