The JOVI.1 monoclonal antibody recognizes an epitope common to a large proportion of human CD4+ or CD8+ T lymphocytes that express the T cell receptor beta chain (TCRβ). The antibody was generated from a mouse immunized with transgenic mouse lymphoid cells that expressed the rearranged human Vβ3-Cβ1 TCR chain derived from the cloned human HA1.7 T helper cell. This antibody reacts with TCR-Cβ1+ T cells and one of several different TCRβ V regions, but not with TCR-Cβ2+ T cells. JOV1.1 antibody reportedly recognized several Cβ1 TCR expressing cell lines or clones including Jurkat, CH7C17, and HA1.7 cells. The JOVI.1 antibody can be used to stimulate proliferative responses by JOVI.1-positive T cells. It can also reportedly be used for immunoprecipitation and to stain JOVI.1+ T cells in frozen tissue sections.
The antibody was conjugated to BD Horizon BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. With an Ex Max near 348 nm and an Em Max near 395 nm, BD Horizon BUV395 can be excited by the ultraviolet laser (355 nm) laser and detected with a 379/28 filter. This dye has been exclusively developed by BD Biosciences as an optimal dye for use on instruments equipped with the ultraviolet laser and has virtually no spillover into any other detector.