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      Purified Rat IgM, κ Isotype Control
      Product Details
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      BD Pharmingen™
      Rat IgM, κ
      Pooled Mouse Ig
      ELISA, Flow cytometry, Isotype control (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Immunohistochemistry-zinc-fixed (Tested During Development)
      250 µg/ml
      AB_479597
      Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Recommended Assay Procedures

      This antibody has been tested by ELISA (~2 μg/ml), and Flow cytometry to assure specificity and reactivity.

      For immunohistochemical staining of acetone-fixed frozen sections or paraffin embeded , a dilution range of 1:12.5 - 1:50 is suggested.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      550342 Rev. 2
      Antibody Details
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      R4-22

      The R4-22 monoclonal antibody has a Rat IgM, κ isotype and has no known specificity.

      550342 Rev. 2
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      550342 Rev.2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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