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      RY610 Rat Anti-Mouse Folate Receptor β (FRβ)

      BD OptiBuild™ RY610 Rat Anti-Mouse Folate Receptor β (FRβ)

      Clone 10/FR-β (also known as 10/FR2; Clone 10/FR2; CL10)

      (RUO)
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      Product Details
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      BD OptiBuild™
      FR-beta; Folbp2; Folr2; folate binding protein 2; folate receptor beta
      Mouse (Tested in Development)
      Rat Wistar IgG2a, κ
      Folate Receptor β-transfected RBL2H3 Cells
      Flow cytometry (Qualified)
      0.2 mg/ml
      14276
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      3. For U.S. patents that may apply, see bd.com/patents.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      7. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. An isotype control should be used at the same concentration as the antibody of interest.
      10. CF™ is a trademark of Biotium, Inc.
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      759696 Rev. 2
      Antibody Details
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      10/FR-β

      The 10/FR-β monoclonal antibody (aka, Clone 10/FR2 or CL10) specifically recognizes mouse Folate Receptor beta (Folate Receptor β or FRβ), a member of the folate receptor family. This cell surface receptor is a glycosylphosphatidylinositol (GPI)-linked membrane protein that is encoded by Folr2 which belongs to the folate-binding receptor family. The Folate Receptor β (FRβ) binds to folate and folic acid derivatives and transports them into cells that are essential for methionine and DNA synthesis which support cell growth and proliferation. Folate Receptor β (FRβ) is expressed on placental cells and on monocytes as well as macrophages found in normal and diseased tissues, eg, tumor-associated macrophages (TAMs) in tumors. Soluble forms of Folate Receptor β (FRβ) have also been described which can be found in various body fluids.

      759696 Rev. 2
      Format Details
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      RY610
      The BD Horizon RealYellow™ 610 (RY610) Dye is part of the BD® family of yellow-green dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 557-nm and an emission maximum (Em Max) at 610-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RY610 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY610 can be used as an alternative to PE-CF594 and we recommend using an optical filter centered near 610-nm (eg, a 610/20-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE-CF594.
      altImg
      RY610
      Yellow-Green 561 nm
      557 nm
      610 nm
      759696 Rev.2
      Citations & References
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      View product citations for antibody "759696" on CiteAb

      Development References (5)

      1. Nagai T, Tanaka M, Tsuneyoshi Y, et al. Targeting tumor-associated macrophages in an experimental glioma model with a recombinant immunotoxin to folate receptor beta.. Cancer Immunol Immunother. 2009; 58(10):1577-86. (Immunogen: Flow cytometry). View Reference
      2. Rodriguez-Garcia A, Lynn RC, Poussin M, et al. CAR-T cell-mediated depletion of immunosuppressive tumor-associated macrophages promotes endogenous antitumor immunity and augments adoptive immunotherapy.. Nat Commun. 2021; 12(1):877. (Clone-specific: Flow cytometry). View Reference
      3. Roy AG, Robinson JM, Sharma P, et al. Folate Receptor Beta as a Direct and Indirect Target for Antibody-Based Cancer Immunotherapy.. Int J Mol Sci. 2021; 22(11):5572. (Clone-specific: Flow cytometry). View Reference
      4. Tie Y, Zheng H, He Z, et al. Targeting folate receptor β positive tumor-associated macrophages in lung cancer with a folate-modified liposomal complex.. Signal Transduct Target Ther. 2020; 5(1):6. (Clone-specific: Flow cytometry). View Reference
      5. Utz SG, See P, Mildenberger W, et al. Early Fate Defines Microglia and Non-parenchymal Brain Macrophage Development.. Cell. 2020; 181(3):557-573.e18. (Clone-specific: Flow cytometry). View Reference
      View All (5) View Less
      759696 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.