Skip to main content Skip to navigation
RY586 Rat Anti-Mouse CD98
RY586 Rat Anti-Mouse CD98
Flow cytometric analysis using BD OptiBuild™ RY586 Rat Anti-Mouse CD98 antibody (Cat. No. 753861; solid line histogram) on viable BALB/c mouse splenocytes stimulated by ConA for 2 days, with corresponding Ig Isotype Control (Cat. No. 568130). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Flow cytometric analysis using BD OptiBuild™ RY586 Rat Anti-Mouse CD98 antibody (Cat. No. 753861; solid line histogram) on viable BALB/c mouse splenocytes stimulated by ConA for 2 days, with corresponding Ig Isotype Control (Cat. No. 568130). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Product Details
Down Arrow Up Arrow


BD OptiBuild™
Cd98; CD98 heavy chain; Slc3a2; 4F2hc; 4F2; Ly-10; Ly-m10; Ly10; Mdu1
Mouse (Tested in Development)
Rat IgG2a, κ
Not Reported
Flow cytometry (Qualified)
0.2 mg/ml
17254
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Researchers should determine the optimal concentration of this reagent for their individual applications.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. CF™ is a trademark of Biotium, Inc.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
753861 Rev. 2
Antibody Details
Down Arrow Up Arrow
H202-141

The H202-141 monoclonal antibody specifically reacts with CD98 (4F2, Ly-10), a heterodimer composed of an 80-kDa glycosolated heavy chain covalently linked to a 34-kDa light chain, which is reported to be involved in amino acid transport.  H202-141 mAb detects high levels of antigen expression on activated lymphocytes and established cell lines and lower levels of expression on resting leukocytes.

753861 Rev. 2
Format Details
Down Arrow Up Arrow
RY586
The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
altImg
RY586
Yellow-Green 561 nm
564 nm
586 nm
753861 Rev.2
Citations & References
Down Arrow Up Arrow
View product citations for antibody "753861" on CiteAb

Development References (4)

  1. Chan MM, Hammerling U, Stutman O. The Ly-10 antigen is a marker of mouse-activated T lymphocytes. Immunogenetics. 1988; 28(6):425-432. (Biology). View Reference
  2. Nakamura E, Sato M, Yang H, et al. 4F2 (CD98) heavy chain is associated covalently with an amino acid transporter and controls intracellular trafficking and membrane topology of 4F2 heterodimer. J Biol Chem. 1999; 274(5):3009-3016. (Biology). View Reference
  3. Ross R, Endlich A, Kumpf K, Schwing J, Reske-Kunz AB. Maturation of epidermal Langerhans cells in vitro is accompanied by downregulation of 4F2 (CD98) as determined by differential display.. J Invest Dermatol. 1998; 110(1):57-61. (Clone-specific: Flow cytometry). View Reference
  4. Sato Y, Heimeier RA, Li C, Deng C, Shi YB. Extracellular domain of CD98hc is required for early murine development.. Cell Biosci. 2011; 1(1):7. (Clone-specific: Flow cytometry). View Reference
View All (4) View Less
753861 Rev. 2

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.