Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
- When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
Companion Products
The 107-1A4 monoclonal antibody specifically recognizes human Prostate-Specific Membrane Antigen (PSMA) which is also known as Glutamate carboxypeptidase II (GCPII), and N-acetyl-L-aspartyl-L-glutamate peptidase I (NAALADase I). PSMA is an ~100 kDa type II transmembrane glycoprotein. It is comprised of a large extracellular region with protease, apical and C-terminal domains that function in substrate binding and enzymatic activity, a transmembrane region, and a short intracellular N-terminal domain. PSMA is encoded by FOLH1 (Folate hydrolase 1) which belongs to the M28 peptidase family. PSMA is expressed in the brain and by cells in various tissues including the prostate, intestines, testis, bladder, liver, kidney, breast, and ovary. In the intestines, this enzyme functions as a folate hydrolase that cleaves glutamate from dietary folates to facilitate the cellular uptake of folic acid. Through its NAALADase activity, this enzyme also acts in the nervous system to hydrolyze the N-aceylaspartylglutamate (NAAG) neuropeptide into NAA and glutamate. This enzyme can thereby modulate excitatory neurotransmission with the release of glutamate which serves as an excitatory neurotransmitter. Overexpression of PSMA by cancer cells, such as prostate cancer cells, may allow for the increased uptake of folates required for rapid cellular division leading to tumor progression. PSMA expression has also been found by some urothelial adenocarcinoma cells and associated with the tumor neovasculature of some tumors as well. The 107-1A4 antibody reportedly recognizes a distinct conformational epitope in the PSMA extracellular domain.
Development References (5)
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Brown LG, Wegner SK, Wang H, et al. A novel monoclonal antibody 107-1A4 with high prostate specificity: generation, characterization of antigen expression, and targeting of human prostate cancer xenografts.. Prostate Cancer Prostatic Dis. 1998; 1(4):208-215. (Immunogen: ELISA, Immunofluorescence, Immunoprecipitation). View Reference
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Chauchereau A, Al Nakouzi N, Gaudin C, et al. Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer.. Exp Cell Res. 2011; 317(3):262-75. (Clone-specific: Flow cytometry). View Reference
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Mhawech-Fauceglia P, Zhang S, Terracciano L, et al. Prostate-specific membrane antigen (PSMA) protein expression in normal and neoplastic tissues and its sensitivity and specificity in prostate adenocarcinoma: an immunohistochemical study using mutiple tumour tissue microarray technique.. Histopathology. 2007; 50(4):472-83. (Biology: Immunohistochemistry). View Reference
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Tykvart J, Navrátil V, Sedlák F, et al. Comparative analysis of monoclonal antibodies against prostate-specific membrane antigen (PSMA).. Prostate. 2014; 74(16):1674-90. (Clone-specific: Flow cytometry, Immunohistochemistry). View Reference
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Wang S, Diamond DL, Hass GM, Sokoloff R, Vessella RL. Identification of prostate specific membrane antigen (PSMA) as the target of monoclonal antibody 107-1A4 by proteinchip; array, surface-enhanced laser desorption/ionization (SELDI) technology.. Int J Cancer. 2001; 92(6):871-6. (Clone-specific: Array). View Reference
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