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Purified Mouse Anti-Human IL-1α
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Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
recombinant human IL-1α
ELISA Capture (Routinely Tested), Intracellular block/flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)
1.0 mg/ml
AB_394101
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

ELISA Capture: Purified 364-3B3-14 antibody can be used as the capture antibody in a sandwich ELISA for measuring human IL-1α protein levels in conjunction with the biotinylated anti-human IL-1a antibody  as the detecting antibody and recombinant human IL-1α as the standard. This purified capture antibody should be pretitrated in the range of 2-6 µg/ml to determine its optimal concentration for ELISA.

Immunofluorescent Staining and Flow Cytometric Analysis: The purified format of 364-3B3-14 clone (Cat. No. 551223) is useful as a blocking control when used with the conjugated form of the antibody. The conjugated format of 364-3B3-14 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify and enumerate IL-1α producing cells within mixed cell populations. PE-conjugated 364-3B3-14 antibodies (Cat. No. 554561) is especially suitable for these studies.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
551223 Rev. 3
Antibody Details
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364-3B3-14

The 364-3B3-14 antibody reacts with human interleukin-1α (IL-1α). The immunogen used to generate the 364-3B3-14 hybridoma was recombinant human IL-1α. The 364-3B3-14 antibody does not cross-react with human IL-1β. This is a non-neutralizing antibody.

551223 Rev. 3
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
551223 Rev.3
Citations & References
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View product citations for antibody "551223" on CiteAb

Development References (2)

  1. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block). View Reference
  2. Thorpe R, Wadhwa M, Gearing A, Mahon B, Poole S. Sensitive and specific immunoradiometric assays for human interleukin-1 alpha. Lymphokine Res. 1988; 7(2):119-127. (Clone-specific). View Reference
551223 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.