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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Companion Products
The YN1/1.7.4 monoclonal antibody specifically binds to CD54 which is also known as Intercellular adhesion molecule 1 (ICAM-1). CD54 (ICAM-1) is a ~90-110 kDa single-pass type I transmembrane glycoprotein that is encoded by Icam1 which belongs to the Immunoglobulin gene superfamily (IgSF). This adhesion molecule is comprised of five IgC2-like domains in its extracellular region, a transmembrane segment, and a cytoplasmic tail. It is expressed on T and B lymphocytes, monocytes, macrophages, dendritic cells (DC), endothelial cells, and epithelial cells. Through binding to cell surface ligands including LFA1 (CD11a/CD18) and Mac-1 (CD11b/CD18), CD54 (ICAM-1) serves to mediate intercellular connections between T cells, B cells, endothelial cells, antigen-presenting cells, and target cells. Its expression is upregulated upon stimulation by inflammatory mediators including proinflammatory cytokines such as IL-1, IFN-γ, and TNF as well as lipopolysaccharide (LPS). CD54 (ICAM-1) participates in leucocyte trans-endothelial migration and trafficking, inflammatory reactions, and antigen-specific immune responses. The YN1/1.7.4 antibody reportedly blocks in vitro and in vivo intercellular adhesion functions mediated by CD54 (ICAM-1) involved in inflammation and immune responses.
Development References (3)
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Horley KJ, Carpenito C, Baker B, Takei F. Molecular cloning of murine intercellular adhesion molecule (ICAM-1).. EMBO J. 1989; 8(10):2889-96. (Clone-specific: Blocking, Flow cytometry, Functional assay, Immunoaffinity chromatography, Inhibition). View Reference
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Takei F. Inhibition of mixed lymphocyte response by a rat monoclonal antibody to a novel murine lymphocyte activation antigen (MALA-2).. J Immunol. 1985; 134(3):1403-7. (Immunogen: Flow cytometry, Functional assay, Immunoprecipitation, Inhibition, Radioimmunoassay). View Reference
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Wang Q, Zhang M, Ding G, et al. Anti-ICAM-1 antibody and CTLA-4Ig synergistically enhance immature dendritic cells to induce donor-specific immune tolerance in vivo.. Immunol Lett. 2003; 90(1):33-42. (Clone-specific: In vivo exacerbation). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.