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Multicolor flow cytometric analysis of CD182 (CXCR2) expression on Mouse myeloid cells. Bone marrow cells from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with BD Horizon™ R718 Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat. No. 567359) and with either BD Horizon™ PE-CF594 Rat IgG2b, κ Isotype Control (Cat. No. 562308; Left Plot) or BD Horizon™ PE-CF594 Rat Anti-Mouse CD182 (CXCR2) antibody (Cat. No. 568211/568212; Right Plot) at 0.5 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density dot plot showing the correlated expression of CD182 (CXCR2) [or Ig Isotype control staining] versus Ly-6C and Ly-6G was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) bone marrow leucocytes. Flow cytometric analysis and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
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BD Horizon™ PE-CF594 Rat Anti-Mouse CD182 (CXCR2)
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The V48-2310 monoclonal antibody specifically recognizes CD182 which is also known as C-X-C chemokine receptor type 2 (CXCR2). CD182 is a seven-transmembrane protein and member of the G protein-coupled receptor 1 family that is encoded by Cxcr2 [Chemokine (C-X-C motif) receptor 2]. CD182 is highly expressed on neutrophils and binds to CXCL1 (KC/GRO-alpha), CXCL2 (MIP2), CXCL3 (Dcip1), CXCL5 (LIX), and CXCL7 (NAP-2). CD182 plays a role in neutrophil activation and trafficking.

Development References (4)
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Bachelerie F, Ben-Baruch A, Burkhardt AM, et al. International Union of Basic and Clinical Pharmacology. [corrected]. LXXXIX. Update on the extended family of chemokine receptors and introducing a new nomenclature for atypical chemokine receptors.. Pharmacol Rev. 2014; 66(1):1-79. (Biology). View Reference
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Barkaway A, Rolas L, Joulia R, et al. Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage.. Immunity. 2021; 54(7):1494-1510.e7. (Biology). View Reference
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Bozic CR, Gerard NP, von Uexkull-Guldenband C, et al. The murine interleukin 8 type B receptor homologue and its ligands. Expression and biological characterization.. J Biol Chem. 1994; 269(47):29355-8. (Biology). View Reference
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Griffith JW, Sokol CL, Luster AD. Chemokines and chemokine receptors: positioning cells for host defense and immunity.. Annu Rev Immunol. 2014; 32:659-702. (Biology). View Reference
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