The 9C11G4 monoclonal antibody specifically binds to CD314, also known as NKG2-D, NKR-P2 and NKLLR. CD314 is encoded by Klrk1 (Killer cell lectin-like receptor subfamily K, member 1). It is a type II transmembrane glycoprotein of the C-type lectin family. This lectin-like receptor is expressed by several cell types including NK cells, NKT cells, γδ T lymphocytes and CD8-positive αβ T lymphocytes. CD314 acts as an activating receptor that can induce NK effector cell activities. NK cells can reportedly bind to members of the RAE1 family, including cell surface RAE-1L and RRLT as ligands, through their CD314 receptors. NK cells are thereby induced to secrete IFN-γ and exert cytotoxicity that may play a role in allograft rejection.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.