The 2.11 monoclonal antibody recognizes the Vγ1.1 chain of the heterodimeric γδ T-cell receptor (TCR γδ) expressed by T lymphocytes and NK-T cells. It does not react with TCR αβ-bearing T cells. TCR γδ associates with CD3 molecules to form the TCR γδ/CD3 complex that mediates antigen recognition and can transduce intracellular signals leading to T cell responses. The Vγ1.1 TCR is also known as Vγ1 TCR. The Vγ1.1 variable region TCR gene segment primarily rearranges to join the Jγ4 and Cγ4 TCR gene segments. TCR Vγ1-expressing cells represent a major population of TCR γδ cells in the adult mouse thymus, peripheral lymphoid tissues, and different epithelia. They constitute a minor population of TCR γδ-positive T cells during fetal and early postnatal life.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.