The MR11-1 monoclonal antibody specifically recognizes the Vβ 12 T-cell Receptor (TCR) of mice having the b haplotype (e.g., C57BL, C58, DBA/1) of the Tcrb gene complex1 The Tcrb-V12 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vβ 12 TCR-bearing T lymphocytes are clonally eliminated in mice expressing I-E and superantigens encoded by Mtv-8 (Mlsf, Dvb11.1), Mtv-9 (Etc-1, Mlsf, Dvb11.2) and/or Mtv-11 (Mlsf, Dvb11.3) proviruses (eg, A, AKR, BALB/c, CBA/J, C3H, DBA/2). Activation of Vβ 12 TCR-expressing T cells by these determinants is dependent upon presentation by I-E. C57BL/6 spleen T cells expressing Vβ 12 TCR are among the predominant responders to MAIDS virus superantigen.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.