The MHN3-21 monoclonal antibody specifically binds to an extracellular domain of human Notch3. Notch3 is a type 1 transmembrane glycoprotein receptor and member of the Notch family that includes Notch1-Notch4. The Notch3 precursor is cleaved in the Golgi and presents as a cell surface heterodimeric receptor. The Notch3 receptor can bind to several membrane-bound ligands including Jagged1, Jagged2 and Delta1. Upon ligand binding, Notch3 undergoes proteolytic cleavage that results in the release of the Notch intracellular domain, NICD. NICD translocates to the nucleus where it can form a transcriptional activator complex with various transcription factors. These multimeric complexes either positively or negatively regulate the expression of multiple genes including those that orchestrate many facets of embryonic development and the subsequent functioning of organ systems such as the hematopoietic, immune, nervous and cardiovascular systems. Notch3 is reportedly expressed by monocytes but not by CD34+ cells and most types of normal lymphocytes. Notch3 has also been implicated in the development of T cell neoplasia. Notch3 is expressed in vascular smooth muscle cells and plays a key role in neural development. Mutations in Notch3 have been identified as the underlying cause of CADASIL, a cerebral autosomal dominant disease that is the most common form of hereditary stroke disorder.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.