The N6B6 monoclonal antibody specifically recognizes CD164. CD164 is a 80-90 kDa, mucin-like molecule present on CD34+ cells during the early stages of B cell, erythroid cell and myelomonocytic cell development. It is also expressed on epithelial cells, peripheral blood monocytes and weakly on lymphocytes. Mucin-like molecules are a new family of glycoproteins present in tissues of the hematopoietic system. They are highly glycosylated polypeptides, containing predominantly O-linked carbohydrate side chains. CD164 may reportedly play a role in hematopoiesis by facilitating the adhesion of CD34+ cells to bone marrow stroma.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.