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BV421 Mouse Anti-Human CD368
BV421 Mouse Anti-Human CD368

Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human CD368 antibody (Cat. No. 744885) on human peripheral blood. Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.

Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human CD368 antibody (Cat. No. 744885) on human peripheral blood. Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.

Product Details
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BD OptiBuild™
CLEC4D; CLECSF8; MCL; CLEC6
Human (Tested in Development)
Mouse IgG2b, κ
Human CD368 Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
X 10-78
AB_2742562
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
744885 Rev. 2
Antibody Details
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9B9

The 9B9 monoclonal antibody specifically binds to CD368, which is also known as CLEC6 (C-type lectin-like receptor 6), CLEC4D, CLECSF8, and MCL (macrophage C-type lectin). CD368 is a 30 kDa type II transmembrane glycoprotein that belongs to the C-type Lectin Receptor family of molecules. It is primarily expressed on peripheral blood neutrophils and monocytes and weakly on several subsets of blood dendritic cells. Expression of this receptor is lost upon in vitro differentiation of monocytes into dendritic cells or macrophages. CD368 reportedly serves as an endocytic receptor that may function in antigen clearance and presentation to T lymphocytes. CD368 may also play roles in cellular adhesion, signaling and glycoprotein turnover. CD368 expression can be upregulated by cells in response to cytokines including IFN-γ, TNF, IL-6, and IL-10.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

744885 Rev. 2
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
744885 Rev.2
Citations & References
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Development References (5)

  1. Arce I, Martínez-Muñoz L, Roda-Navarro P, Fernández-Ruiz E. The human C-type lectin CLECSF8 is a novel monocyte/macrophage endocytic receptor.. Eur J Immunol. 2004; 34(1):210-20. (Biology). View Reference
  2. Autenrieth SE, Grimm S, Rittig SM, Grünebach F, Gouttefangeas C, Bühring HJ. Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 Workshop in Wollongong, Australia.. Clin Transl Immunology. 2015; 4(11):e50. (Clone-specific: Flow cytometry). View Reference
  3. Graham LM, Gupta V, Schafer G, et al. The C-type lectin receptor CLECSF8 (CLEC4D) is expressed by myeloid cells and triggers cellular activation through Syk kinase.. J Biol Chem. 2012; 287(31):25964-74. (Biology). View Reference
  4. Gurka S, Dirks S, Photiadis J, Kroczek RA. Expression analysis of surface molecules on human thymic dendritic cells with the 10th HLDA Workshop antibody panel.. Clin Transl Immunology. 2015; 4(10):e47. (Clone-specific). View Reference
  5. Ohradanova-Repic A, Machacek C, Fischer MB, Stockinger H. Differentiation of human monocytes and derived subsets of macrophages and dendritic cells by the HLDA10 monoclonal antibody panel.. Clin Transl Immunology. 2016; 5(1):e55. (Clone-specific: Flow cytometry). View Reference
View All (5) View Less
744885 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.