The 421203 monoclonal antibody specifically recognizes Claudin-1 which is also known as Senescence-associated epithelial membrane protein 1 (SEMP1) or Ichthyosis, leukocyte vacuoles, alopecia, and sclerosing cholangitis (ILVASC). Claudin-1 is a ~23 kDa multi-pass membrane protein that is encoded by CLDN1 (claudin 1) which belongs to the claudin family. Claudin-1 is expressed by Langerhans cells, dendritic cells, epithelial cells and endothelial cells. It serves as a major component of tight junctions between epithelial or endothelial cells. Claudin-1 may also function as a co-receptor for Hepatitis C virus (HCV) entry into hepatic cells. Dysregulated Claudin-1 expression has been detected in multiple types of cancer.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.