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      Biotin Armenian Hamster Anti-ICOS (CD278)
      Biotin Armenian Hamster Anti-ICOS (CD278)
      Multicolor Flow Cytometric Analysis of ICOS (CD278) Expression    Panel 1- ICOS (CD278) expression on Human peripheral blood lymphocytes (PBL). Human whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD3 antibody (Cat. No. 562426/562427) and either no antibody (Autofluorescence Control; Top Plot) or Biotin Armenian Hamster Anti-ICOS (CD278) antibody (Cat. No. 568036; Bottom Plot) at 0.5 µg/test. The cells were counterstained with PE-Streptavidin (Cat. No. 554061). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The pseudocolor density plot showing the correlated expression of ICOS (CD278) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.    Panel 2- ICOS (CD278) expression on Mouse thymocyte subsets. Mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with BD Horizon™ R718 Rat Anti-Mouse CD4 (Cat. No. 566939) and BD Horizon™ BUV615 Rat Anti-Mouse CD8a (Cat. No. 613004) antibodies, and with either no antibody (Autofluorescence Control; dashed line histograms) or Biotin Armenian Hamster Anti-ICOS (CD278) antibody (solid line histograms) at 0.5 µg/test. The cells were counterstained with PE-Streptavidin (Cat. No. 554061). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing ICOS (CD278) expression (or Autofluorescence) were derived from CD4 and CD8a gated events as indicated with the forward and side light-scatter characteristics of viable (DAPI-negative) thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Biotin Armenian Hamster Anti-ICOS (CD278)
      Multicolor Flow Cytometric Analysis of ICOS (CD278) Expression    Panel 1- ICOS (CD278) expression on Human peripheral blood lymphocytes (PBL). Human whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD3 antibody (Cat. No. 562426/562427) and either no antibody (Autofluorescence Control; Top Plot) or Biotin Armenian Hamster Anti-ICOS (CD278) antibody (Cat. No. 568036; Bottom Plot) at 0.5 µg/test. The cells were counterstained with PE-Streptavidin (Cat. No. 554061). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The pseudocolor density plot showing the correlated expression of ICOS (CD278) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.    Panel 2- ICOS (CD278) expression on Mouse thymocyte subsets. Mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with BD Horizon™ R718 Rat Anti-Mouse CD4 (Cat. No. 566939) and BD Horizon™ BUV615 Rat Anti-Mouse CD8a (Cat. No. 613004) antibodies, and with either no antibody (Autofluorescence Control; dashed line histograms) or Biotin Armenian Hamster Anti-ICOS (CD278) antibody (solid line histograms) at 0.5 µg/test. The cells were counterstained with PE-Streptavidin (Cat. No. 554061). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing ICOS (CD278) expression (or Autofluorescence) were derived from CD4 and CD8a gated events as indicated with the forward and side light-scatter characteristics of viable (DAPI-negative) thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      ICOS; AILIM; H4; Inducible T-cell costimulator; CVID1; Ly115
      Human (QC Testing), Mouse (Tested in Development), Rat, Rhesus (Reported)
      Armenian Hamster IgG
      Mouse D10.G4.1 T-cell clone
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
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      568036 Rev. 1
      Antibody Details
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      C398.4A

      The C398.4A monoclonal antibody specifically binds to Inducible Costimulator (ICOS), which is also known as, CD278, Activation-inducible lymphocyte immunomediatory molecule (AILIM), or H4. ICOS is a type I transmembrane glycoprotein that forms a disulfide-linked homodimer and belongs to the CD28 family within the Ig superfamily. ICOS is expressed on either CD4+ or CD8+ single-positive mature thymocytes, T cells, or subsets of Innate Lymphoid Cells (ILC). Its expression is upregulated on activated T lymphocytes. ICOS is a costimulatory receptor that can bind to the B7-H2 ligand (CD275, ICOS-L) that is expressed on B cells, monocytes, macrophages, dendritic cells, and endothelial cells. ICOS plays a critical role in many types of T cell-dependent immunity. In the case of humoral immunity, for example, ICOS signaling is critical for the differentiation of T follicular helper (Tfh) cells and development of germinal centers. Although C398.4A was generated against mouse ICOS, this antibody reportedly crossreacts with human, rhesus, and rat ICOS.

      568036 Rev. 1
      Format Details
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      568036 Rev.1
      Citations & References
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      View product citations for antibody "568036" on CiteAb

      Development References (11)

      1. Araujo LM, Fert I, Jouhault Q, et al. Increased production of interleukin-17 over interleukin-10 by treg cells implicates inducible costimulator molecule in experimental spondyloarthritis. Arthritis Rheum. 2014; 66(9):2412-2422. (Clone-specific: Flow cytometry). View Reference
      2. Brenchley JM, Vinton C, Tabb B, et al. Differential infection patterns of CD4+ T cells and lymphoid tissue viral burden distinguish progressive and nonprogressive lentiviral infections.. Blood. 2012; 120(20):4172-81. (Clone-specific: Flow cytometry). View Reference
      3. Buonfiglio D, Bragardo M, Bonissoni S, et al. Characterization of a novel human surface molecule selectively expressed by mature thymocytes, activated T cells and subsets of T cell lymphomas. Eur J Immunol. 1999; 29(9):2863-2879. (Clone-specific: Flow cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay). View Reference
      4. Buonfiglio D, Bragardo M, Redoglia V, et al. The T cell activation molecule H4 and the CD28-like molecule ICOS are identical. Eur J Immunol. 2000; 30(12):3463-3467. (Clone-specific: Blocking, Flow cytometry). View Reference
      5. Chen Y, Shen S, Gorentla BK, Gao J, Zhong XP. Murine regulatory T cells contain hyperproliferative and death-prone subsets with differential ICOS expression. J Immunol. 2012; 188(4):1698-1707. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
      6. Hutloff A, Dittrich AM, Beier KC, et al. ICOS is an inducible T-cell co-stimulator structurally and functionally related to CD28. Nature. 1999; 397(3716):263-266. (Biology). View Reference
      7. Klatt NR, Vinton CL, Lynch RM et al. SIV infection of rhesus macaques results in dysfunctional T- and B-cell responses to neo and recall Leishmania major vaccination. Blood. 2011; 118(22):5803-5812. (Clone-specific: Flow cytometry). View Reference
      8. McAdam AJ, Chang TT, Lumelsky AE, et al. Mouse inducible costimulatory molecule (ICOS) expression is enhanced by CD28 costimulation and regulates differentiation of CD4+ T cells.. J Immunol. 2000; 165(9):5035-40. (Biology). View Reference
      9. Redoglia V, Dianzani U, Rojo JM, et al. Characterization of H4: a mouse T lymphocyte activation molecule functionally associated with the CD3/T cell receptor. Eur J Immunol. 1996; 26(11):2781-2789. (Immunogen: Bioassay, (Co)-stimulation, Flow cytometry, Functional assay, Immunoprecipitation, Radioimmunoassay). View Reference
      10. Sonnenberg GF, Mjosberg J, Spits H, Artis D. SnapShot: innate lymphoid cells. Immunity. 2013; 39(3):622-623. (Biology). View Reference
      11. Xu H, Wang X, Lackner AA, Veazey RS. PD-1(HIGH) Follicular CD4 T Helper Cell Subsets Residing in Lymph Node Germinal Centers Correlate with B Cell Maturation and IgG Production in Rhesus Macaques. Front Biosci. 2014; 5(85):1-7. (Clone-specific: Flow cytometry). View Reference
      View All (11) View Less
      568036 Rev. 1

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      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.