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      BB700 Rat Anti-Mouse Blimp-1
      BB700 Rat Anti-Mouse Blimp-1
      Multicolor flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide LPS) and stained with BD Horizon™ BV421 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 562922). The cells were subsequently fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either BD Horizon™ BB700 Rat IgG2a, κ Isotype Control (Cat. No. 566413; Left Plot) or BD Horizon™ BB700 Rat Anti-Mouse Blimp-1 antibody (Cat. No. 567830; Right Plot) at 0.25 µg/test. The bivariate pseudocolor density plot showing the correlated expression of Blimp-1 (or Ig Isotype control staining) versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of intact activated leucocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype Control-stained cells (data not shown). Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      BB700 Rat Anti-Mouse Blimp-1
      Multicolor flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide LPS) and stained with BD Horizon™ BV421 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 562922). The cells were subsequently fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either BD Horizon™ BB700 Rat IgG2a, κ Isotype Control (Cat. No. 566413; Left Plot) or BD Horizon™ BB700 Rat Anti-Mouse Blimp-1 antibody (Cat. No. 567830; Right Plot) at 0.25 µg/test. The bivariate pseudocolor density plot showing the correlated expression of Blimp-1 (or Ig Isotype control staining) versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of intact activated leucocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype Control-stained cells (data not shown). Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Horizon™
      Prdm1; PR domain zinc finger protein 1; Blimp1; PRDI-BF1; ZNFPR1A1
      Mouse (QC Testing)
      Rat IgG2a, κ
      Mouse Blimp-1 Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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      Recommended Assay Procedures

      For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      7. BD Horizon Brilliant Blue 700 is covered by one or more of the following US patents: 8,455,613 and 8,575,303.
      8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      9. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
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      567830 Rev. 1
      Antibody Details
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      5E7

      The 5E7 monoclonal antibody specifically binds to mouse B lymphocyte-induced maturation protein 1 (Blimp-1). Blimp-1 is a 98 kDa zinc finger-containing protein that is encoded by the Prdm1 gene and functions as a transcriptional repressor. Blimp-1 is essential for regulating the terminal differentiation of activated B cells into plasma cells. In this process, Blimp-1 suppresses genes involved in B cell proliferation, such as Myc and Bcl6. It controls other genes, such as Xbp1, that are required for upregulating the plasma cell's protein synthesis machinery which is essential for antibody production and secretion. Blimp-1 is an important regulator in other cell types as well. It functions in the regulation of T cell activation and homeostasis and NK cell maturation. Blimp-1 is expressed in activated T cells and can function to inhibit IL-2 production while enhancing the differentiation and expression of several molecules produced by effector T cells.

      567830 Rev. 1
      Format Details
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      BB700
      The BD Horizon Brilliant™ Blue 700 (BB700) Dye is part of the BD Horizon Brilliant™ Blue family of dyes. This tandem fluorochrome is comprised of a polymer-technology dye donor with an excitation maximum (Ex Max) of 476-nm and an acceptor dye with an emission maximum (Em Max) at 695-nm. Driven by BD innovation, BB700 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 695-nm (e.g., a 695/20-nm bandpass filter). The donor dye can be excited by the Violet (405 nm) laser and the acceptor dye can be excited by the red (627–640 nm) laser resulting in cross-laser excitation and fluorescence spillover. BB700 Reagents are significantly brighter than equivalent PerCP or PerCP-Cy5.5 reagents and are less sensitive to photobleaching. In addition, BB700 shows much less excitation by the violet (407-nm) laser resulting in less spillover. BB700 has minimal yellow green (562-nm) excitation and is ideal for instruments with both blue (488-nm) and yellow green (562-nm) lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BB700
      Blue 488 nm
      476 nm
      695 nm
      567830 Rev.1
      Citations & References
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      View product citations for antibody "567830" on CiteAb

      Development References (9)

      1. Crotty S, Johnston RJ, Schoenberger SP. Effectors and memories: Bcl-6 and Blimp-1 in T and B lymphocyte differentiation. Nat Immunol. 2010; 11(2):114-120. (Biology). View Reference
      2. Huang S. Blimp-1 is the murine homolog of the human transcriptional repressor PRDI-BF1. Cell. 1994; 78(1):9. (Biology). View Reference
      3. Johnston RJ, Poholek AC, DiToro D, et al. Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation.. Science. 2009; 325(5943):1006-10. (Biology). View Reference
      4. Kallies A, Carotta S, Huntington ND, et al. A role for Blimp1 in the transcriptional network controlling natural killer cell maturation. Blood. 2011; 117(6):1869-1879. (Immunogen: Western blot). View Reference
      5. Kallies A, Hasbold J, Tarlinton DM, et al. Plasma cell ontogeny defined by quantitative changes in blimp-1 expression. J Exp Med. 2004; 200(8):967-977. (Biology). View Reference
      6. Kallies A, Hawkins ED, Belz GT, et al. Transcriptional repressor Blimp-1 is essential for T cell homeostasis and self-tolerance. Nat Immunol. 2006; 7(5):466-474. (Biology). View Reference
      7. Kim SJ, Gregersen PK, Diamond B. Regulation of dendritic cell activation by microRNA let-7c and BLIMP1. J Clin Invest. 2013; 123(2):823-833. (Biology). View Reference
      8. Martins GA, Cimmino L, Liao J, Magnusdottir E, Calame K. Blimp-1 directly represses Il2 and the Il2 activator Fos, attenuating T cell proliferation and survival. J Exp Med. 2008; 205(9):1959-1965. (Biology). View Reference
      9. Turner CA Jr, Mack DH, Davis MM. Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B lymphocytes into immunoglobulin-secreting cells. Cell. 1994; 77(2):297-306. (Biology). View Reference
      View All (9) View Less
      567830 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.