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      Alexa Fluor™ 647 Mouse Anti-Human TKT
      Alexa Fluor™ 647 Mouse Anti-Human TKT

      Flow cytometric analysis of TKT expression in HeLa cells. Cells from the Human HeLa (Cervical adenocarcinoma, ATCC® CRM-CCL-2™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ buffer (Cat. No. 554723). The cells were then stained with Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Cat. No. 557732; dashed line histogram) or Alexa Fluor™ 647 Mouse Anti-Human TKT antibody (Cat. No. 569546; solid line histogram) at 0.125 µg/test. The fluorescence histogram showing TKT expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.

      Alexa Fluor™ 647 Mouse Anti-Human TKT

      Flow cytometric analysis of TKT expression in HeLa cells. Cells from the Human HeLa (Cervical adenocarcinoma, ATCC® CRM-CCL-2™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ buffer (Cat. No. 554723). The cells were then stained with Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Cat. No. 557732; dashed line histogram) or Alexa Fluor™ 647 Mouse Anti-Human TKT antibody (Cat. No. 569546; solid line histogram) at 0.125 µg/test. The fluorescence histogram showing TKT expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.

      Product Details
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      BD Pharmingen™
      TK; TKT1; epididymis luminal protein 107; epididymis secretory protein Li 48; transketolase
      Human (QC Testing)
      Mouse IgG1, κ
      Not Reported
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      7086
      AB_3685143
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
      8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      10. This product is, in part, provided under an intellectual property license from Abcam. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product for buyer’s internal research use only (whether the buyer is an academic or for-profit entity) and buyer shall not be permitted to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, process, make-up or production of this product (or any portion thereof). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. If you, as the buyer, do not agree to these conditions, please leave the product unopened and return the contents of this package to BD Biosciences, 10975 Torreyana Road, San Diego, CA 92121. For information on purchasing a license to this product for purposes other than research, contact Abcam at partnerships@abcam.com.
      11. For U.S. patents that may apply, see bd.com/patents.
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      569546 Rev. 2
      Antibody Details
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      7H1AA1

      The 7H1AA1 monoclonal antibody specifically recognizes Transketolase (TKT), also known as TK or TKT1. TKT is a ~68 kDa ubiquitously expressed enzyme encoded by the TKT gene, which belongs to the transketolase family. Located in the cytoplasm, TKT is a critical thiamine-dependent enzyme involved in the interaction between glycolysis and the non-oxidative branch of the penthose phosphate pathway (PPP) of carbohydrate metabolism, which allows cells to adapt to different metabolic needs.  TKT expression within tissues is consequently linked to central metabolic function, making TKT a critical determinant for many biological processes. TKT reportedly plays a pivotal role in the regulation of cancer and may be a promising drug target.

      569546 Rev. 2
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      569546 Rev.2
      Citations & References
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      View product citations for antibody "569546" on CiteAb

      Development References (6)

      1. Ahl PJ, Hopkins RA, Xiang WW, et al. Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.. Communications Biology. 2020; 3(1):1-15. (Clone-specific: Flow cytometry). View Reference
      2. Hao S, Meng Q, Sun H, et al. The role of transketolase in human cancer progression and therapy.. Biomed Pharmacother. 2022; 154:113607. (Biology). View Reference
      3. Kochetov GA, Solovjeva ON. Structure and functioning mechanism of transketolase.. Biochim Biophys Acta. 2014; 1844(9):1608-18. (Biology). View Reference
      4. Mitschke L, Parthier C, Schröder-Tittmann K, Coy J, Lüdtke S, Tittmann K. The crystal structure of human transketolase and new insights into its mode of action.. J Biol Chem. 2010; 285(41):31559-70. (Biology). View Reference
      5. Schenk G, Duggleby RG, Nixon PF. Properties and functions of the thiamin diphosphate dependent enzyme transketolase.. Int J Biochem Cell Biol. 1998; 30(12):1297-318. (Biology). View Reference
      6. Xu IM, Lai RK, Lin SH, et al. Transketolase counteracts oxidative stress to drive cancer development.. Proc Natl Acad Sci U S A. 2016; 113(6):E725-34. (Biology). View Reference
      View All (6) View Less
      569546 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.