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Alexa Fluor™ 488 Mouse Anti-Human Basophils (2D7)

BD Pharmingen™ Alexa Fluor™ 488 Mouse Anti-Human Basophils (2D7)

Clone 2D7/BASO (also known as 2D7)

(RUO)
Alexa Fluor™ 488 Mouse Anti-Human Basophils (2D7)
Two-color flow cytometric analysis of Basophils (2D7) expression in Human peripheral blood leucocytes. Human whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD193 antibody (Cat. No. 562570/566279) and then fixed with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) and permeabilized with BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885). The cells were then stained with either Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; Left Plot) or Alexa Fluor™ 488 Mouse Anti-Human Basophils (2D7) antibody (Cat. No. 568378/568379; Right Plot). The bivariate pseudocolor density plot showing the correlated expression of Basophils (2D7) [or Ig Isotype control staining] versus CD193 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Alexa Fluor™ 488 Mouse Anti-Human Basophils (2D7)
Multiparameter flow cytometric analysis of Basophils (2D7) expression in Human peripheral blood leucocyte populations. Human whole blood was fixed with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) and permeabilized with BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885). The cells were then stained with either Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; Left Plot) or Alexa Fluor™ 488 Mouse Anti-Human Basophils (2D7) antibody (Cat. No. 568378/568379; Right Plot). The bivariate pseudocolor density plot showing the correlated expression of Basophils (2D7) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Two-color flow cytometric analysis of Basophils (2D7) expression in Human peripheral blood leucocytes. Human whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD193 antibody (Cat. No. 562570/566279) and then fixed with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) and permeabilized with BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885). The cells were then stained with either Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; Left Plot) or Alexa Fluor™ 488 Mouse Anti-Human Basophils (2D7) antibody (Cat. No. 568378/568379; Right Plot). The bivariate pseudocolor density plot showing the correlated expression of Basophils (2D7) [or Ig Isotype control staining] versus CD193 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Multiparameter flow cytometric analysis of Basophils (2D7) expression in Human peripheral blood leucocyte populations. Human whole blood was fixed with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) and permeabilized with BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885). The cells were then stained with either Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; Left Plot) or Alexa Fluor™ 488 Mouse Anti-Human Basophils (2D7) antibody (Cat. No. 568378/568379; Right Plot). The bivariate pseudocolor density plot showing the correlated expression of Basophils (2D7) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Product Details
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BD Pharmingen™
Basophils; Basophils (2D7); 2D7 Antigen; 2D7 Ligand
Human (QC Testing)
Mouse BALB/c IgG1, κ
Purified Human Basophil Lysate
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
  8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
568378 Rev. 1
Antibody Details
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2D7/BASO

The 2D7 monoclonal antibody selectively recognizes human basophils, Basophils (2D7). The antibody binds to a ~7.2 to 7.5 kDa basophil-specific protein, also known as the 2D7 antigen or ligand, present in secretory granules which has not been conclusively identified. Basophils normally comprise a relatively small percentage (<1%) of total peripheral blood leucocytes. Basophils express high levels of FceRI, a high affinity receptor for IgE. Upon activation through crosslinking of FceRI, basophils degranulate and may thus show reduced staining with the 2D7 antibody due to the release or destruction of the 2D7 antigen.  The activated basophils release proinflammatory mediators that can either counter parasitic infections or lead to acute or chronic allergic diseases, including anaphylaxis, asthma, and atopic dermatitis. Activated basophils release a variety of mediators including the vasodilator histamine, proteolytic enzymes, and several cytokines which promote inflammation as well as heparin which  prevents blood clotting. The 2D7 monoclonal antibody is useful for identifying human basophils since it does not recognize lymphocytes, monocytes, eosinophils, neutrophils, or mast cells.

568378 Rev. 1
Format Details
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Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
568378 Rev.1
Citations & References
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View product citations for antibody "568378" on CiteAb

Development References (6)

  1. Agis H, Krauth MT, Mosberger I, et al. Enumeration and immunohistochemical characterisation of bone marrow basophils in myeloproliferative disorders using the basophil specific monoclonal antibody 2D7. J Clin Pathol. 2006; 59(4):396-402. (Clone-specific: Immunohistochemistry). View Reference
  2. Foster B, Schwartz LB, Devouassoux G, Metcalfe DD, Prussin C. Characterization of mast-cell tryptase-expressing peripheral blood cells as basophils. J Allergy Clin Immunol. 2002; 109(2):287-293. (Clone-specific: Flow cytometry). View Reference
  3. Horny HP, Sotlar K, Stellmacher F, et al. The tryptase positive compact round cell infiltrate of the bone marrow (TROCI-BM): a novel histopathological finding requiring the application of lineage specific markers. J Clin Pathol. 2006; 59(3):298-302. (Clone-specific: Immunohistochemistry). View Reference
  4. Karasuyama H, Obata K, Wada T, Tsujimura Y, Mukai K. Newly appreciated roles for basophils in allergy and protective immunity.. Allergy. 2011; 66(9):1133-41. (Clone-specific). View Reference
  5. Kepley CL, Craig SS, Schwartz LB. Identification and partial characterization of a unique marker for human basophils.. J Immunol. 1995; 154(12):6548-55. (Immunogen: Electron microscopy, Immunocytochemistry, Western blot). View Reference
  6. Plager DA, Weiss EA, Kephart GM, et al. Identification of basophils by a mAb directed against pro-major basic protein 1.. J Allergy Clin Immunol. 2006; 117(3):626-34. (Clone-specific: Immunofluorescence, Immunohistochemistry). View Reference
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568378 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.