Description
The BD™ HLA-B27 system is a qualitative two-color direct immunofluorescence method for the rapid detection of HLA-B27 antigen expression in erythrocyte-lysed whole blood (LWB) using the BD FACSCanto™ family of flow cytometers, or the BD FACSCalibur™, BD FACSort™, or BD FACScan™ flow cytometer.
Contents: Anti-HLA-B27 FITC / CD3 PE, HLA-B27 calibration beads, and BD FACS™ Lysing Solution.
Preparation And Storage
• Store Reagent A and Reagent C upright at 2° to 8°C. Do not use after the expiration date shown on the label. • Store Reagent B, undiluted, at 2° to 25°C. When diluted, store at room temperature (20° to 25°C) for up to one month. • Do not freeze the reagents or expose them to direct light during storage or incubation with cells. Keep reagent vials dry.
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| HLA-B27 calibration beads | 50 Tests (1 ea) | 91-0176 | N/A |
| BD FACS™ Lysing Solution | 50 Tests (1 ea) | 91-0175 | N/A |
| Anti-HLA-B27 FITC / CD3 PE | 50 Tests (1 ea) | 91-0177 | N/A |
Development References (14)
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Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology: Flow cytometry).
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Dausset J. The major histocompatibility in man, Past, present, and future concepts. Science. 1981; 213:1469-1474. (Biology: Flow cytometry).
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Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 19863-30.
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Johnson J, Garbutt J, Nelson K. A monoclonal antibody specific for HLA-B27. Human Immunol. 1985; 134-135. (Biology: Flow cytometry).
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Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983; 131:536-539. (Biology: Flow cytometry).
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Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:259-288.
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Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology: Flow cytometry). View Reference
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Levering WH, Wind H, Sintnicolaas K, Hooijkaas H, Gratama JW. Flow cytometric HLA-B27 screening: cross-reactivity patterns of commercially available anti-HLA-B27 monoclonal antibodies with other HLA-B antigens. Cytometry B Clin Cytom. 2003; 54B:28-38. (Biology: Flow cytometry).
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Lopez-Larrea C, Gonzalez-Roces S, Alvarez V. HLA-B27 structure, function, and disease association. Curr Opin Rheumatol. 1996; 8:296-308. (Biology: Flow cytometry).
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Müller H, Albrecht J, Schabel A. New aspects in HLA-typing by use of monoclonal antibodies and calibrated flow cytometry. Fresenius A Anal Chem. 1988; 330:341. (Biology: Flow cytometry).
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NCCLS document. 2001; (Biology: Flow cytometry).
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Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture–Fourth Edition; Approved Standard. NCCLS document H3-A4. 1998; (Biology: Flow cytometry).
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Seipp MT, Erali M, Wies RL, Wittwer C. HLA-B27 typing: evaluation of an allele-specific PCR melting assay and two flow cytometric antigen assays. Cytometry B Clin Cytom. 2005; 63:44119. (Biology: Flow cytometry).
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Taurog JD, El-Zaatari FA. In vitro mutagenesis of HLA-B27. Substitution of an unpaired cysteine residue in the α 1 domain causes loss of antibody-defined epitopes. J Clin Invest. 1988; 82:987-992. (Biology: Flow cytometry).
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For In Vitro Diagnostics Use.
Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.
