Skip to main content Skip to navigation
Purified Mouse Anti-GAP-43/Neuromodulin

BD Transduction Laboratories™ Purified Mouse Anti-GAP-43/Neuromodulin

Clone 31/GAP-43/Neuromodulin

Purified Mouse Anti-GAP-43/Neuromodulin
Western blot analysis of GAP-43/Neuromodulin on rat cerebrum lysate. Lane 1: 1:10000, lane 2: 1:20000, lane 3: 1:40000 dilution of anti-GAP-43/Neuromodulin.
Western blot analysis of GAP-43/Neuromodulin on rat cerebrum lysate. Lane 1: 1:10000, lane 2: 1:20000, lane 3: 1:40000 dilution of anti-GAP-43/Neuromodulin.
Product Details
Down Arrow Up Arrow

BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Human GAP-43/Neuromodulin aa. 1-144
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
43 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
612262 Rev. 1
Antibody Details
Down Arrow Up Arrow

Neuromodulin (GAP-43, B-50, F-1) is a neural-specific protein that represents about one percent of the total membrane protein in growth cones from developing rat brain. Growth cones are located at the distal tip of neuronal processes and are critical for transduction of extracellular signals into directed growth. Neuromodulin is attached to the membranes of the neuronal growth cones during development. In the adult, it is attached to the presynaptic membranes in discrete subsets of synapses. Neuromodulin can bind to calmodulin, as well as inhibit phosphatidylinositol phosphate kinase (PIP kinase) activity. It is also a prominent substrate of Protein Kinase C (PKC) and may mediate some of the effects of PKC on growth cone function. As may be expected, expression and phosphorylation of neuromodulin are closely associated with axonal outgrowth and repair. Phosphorylation of neuromodulin by PKC has been correlated with the persistence of long term potentiation and the translocation of PKC to synaptosomal membranes. This PKC-mediated phosphorylation does not appear to require neuromodulin membrane association. It has also been demonstrated that the GTP-binding protein, G[o], is a main component of growth cone membranes. The binding of GTP--S to G[o] is stimulated by neuromodulin and this activity resides within the amino-terminal region of the protein. This region of neuromodulin shares sequence homology with the cytoplasmic tails of G-linked transmembrane receptors, suggesting the presence of one binding site on G[o] for both neuromodulin and transmembrane receptors.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612262 Rev. 1
Format Details
Down Arrow Up Arrow
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
612262 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (4)

  1. Chapman ER, Estep RP, Storm DR. Palmitylation of neuromodulin (GAP-43) is not required for phosphorylation by protein kinase C. J Biol Chem. 1992; 267(35):25233-25238. (Biology). View Reference
  2. Cimler BM, Andreasen TJ, Andreasen KI, Storm DR. P-57 is a neural specific calmodulin-binding protein. J Biol Chem. 1985; 260(19):10784-10788. (Biology). View Reference
  3. Meiri KF, Bickerstaff LE, Schwob JE. Monoclonal antibodies show that kinase C phosphorylation of GAP-43 during axonogenesis is both spatially and temporally restricted in vivo. J Cell Biol. 1991; 112(5):991-1005. (Biology). View Reference
  4. Strittmatter SM, Valenzuela D, Kennedy TE, Neer EJ, Fishman MC. G0 is a major growth cone protein subject to regulation by GAP-43. Nature. 1990; 344(6269):836-841. (Biology). View Reference
View All (4) View Less
612262 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.