Description
Cell or tissue lysates are a useful control for the western blotting application. A source that can be rich in endogenously expressed proteins, this lysate is convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel.
Preparation And Storage
Mouse macrophages (RAW 264.7 cells) were grown to 70% confluencey, then stimulated with 10 ng/ml IFNγ and 1 µg/ml LPS for 12 hours. Western blots of mouse macrophages stimulated with IFNγ and LPS are evaluated for the induction of iNOS and Cox-2 using monoclonal antibodies Cat. No. 610328 and 610203 respectively. Unstimulated mouse macrophages (Cat. No. 611479) show no expression of iNOS or Cox-2.
Recommended Assay Procedures
Warning: BD Transduction Laboratories™ Mouse Macrophage + IFNγ/LPS Lysate contains 12.171% 1,2,3-Propanetriol and 2.068% sodium dodecyl sulfate (w/w).
Hazard Statements:
Causes serious eye irritation.
Precautionary Statements:
Wear protective gloves/protective clothing/eye protection/face protection.
Wash thoroughly after handling.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
