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Purified Mouse Anti-Human VEGF
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG2a
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

For western blot analysis use at 0.5-2µg/ml.  Detailed protocols are at:

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554539 Rev. 1
Antibody Details
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The G143-850 antibody recognizes vascular endothelial growth factor (VEGF) by western blotting. VEGF is a homodimeric heavily glycosylated protein. Human VEGF occurs in several molecular variants which arise by alternative splicing of the mRNA. Splice forms of VEGF differ in biological properties such as the receptor types which they recognize. Various forms of VEGF can be detected at MW 18-25 kDa in SDS-PAGE.

554539 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
554539 Rev.1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.