Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
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Nuclear receptors (NRs) are a family of ligand-inducible transcription factors that trigger complex events during development, differentiation, and homeostasis. They respond to the binding of steroid and thyroid hormones, retinoids, and vitamins. NRs contain an activation domain AF-1, which constitutively activates transcription. A second activation domain, AF-2, responds to ligand binding. Transcriptional interference/squelching between the AFs of steroid receptors first suggested the existence of Transcriptional Intermediary Factors (TIFs). TIFs mediate AF activity to the transcriptional machinery and chromatin template. TIF2 interacts directly with the ligand binding domains of several NRs in an agonist and AF-2-integrity-dependent manner. It has autonomous activation function, relieves interference between NRs, and has been shown to enhance AF-2 activity. TIF2 contains an NR interaction domain (NID) and two autonomous activation functions (AD1 and AD2). AD1 is identical to the TIF2 CBP interaction domain (CID). Thus, it is thought that TIF2 links NR AF2 activity and CBP via AD1 and functions as a transcriptional mediator through unknown CBP-independent mechanisms via AD2.
Development References (4)
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Heery DM, Kalkhoven E, Hoare S, Parker MG. A signature motif in transcriptional co-activators mediates binding to nuclear receptors. Nature. 1997; 387(6634):733-736. (Biology). View Reference
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Muller JM, Metzger E, Greschik H. The transcriptional coactivator FHL2 transmits Rho signals from the cell membrane into the nucleus. EMBO J. 2002; 21(14):736-748. (Biology: Western blot). View Reference
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Voegel JJ, Heine MJ, Zechel C, Chambon P, Gronemeyer H. TIF2, a 160 kDa transcriptional mediator for the ligand-dependent activation function AF-2 of nuclear receptors. EMBO J. 1996; 15(14):3667-3675. (Biology). View Reference
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Xu J, Qiu Y, DeMayo FJ, Tsai SY, Tsai MJ, O'Malley BW. Partial hormone resistance in mice with disruption of the steroid receptor coactivator-1 (SRC-1) gene. Science. 1998; 279(5358):1922-1925. (Biology). View Reference
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