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Purified Mouse Anti-Rat Tau
Purified Mouse Anti-Rat Tau

Western blot analysis of Tau on a rat cerebrum lysate (left). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Tau antibody.

Immunofluorescent staining of SK-N-SH cells (right).  Cells were seeded in a 384 well collagen coated Microplates (Material # 353962) at ~ 8,000 cells per well.  After overnight incubation, cells were stained using the Triton X100 fix/perm protocol (see Recommended Assay Procedure; Bioimaging protocol link) and the anti- Tau antibody.  The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken on a BD Pathway™  855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison ™ software.  This antibody also stained SH-SY5Y, SK-N-SH, U87 and U373 cells using both the Triton X100 and methanol fix/perm protocols (see Recommended Assay Procedure; Bioimaging protocol link).

Western blot analysis of Tau on a rat cerebrum lysate (left). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Tau antibody.

Immunofluorescent staining of SK-N-SH cells (right).  Cells were seeded in a 384 well collagen coated Microplates (Material # 353962) at ~ 8,000 cells per well.  After overnight incubation, cells were stained using the Triton X100 fix/perm protocol (see Recommended Assay Procedure; Bioimaging protocol link) and the anti- Tau antibody.  The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken on a BD Pathway™  855 or 435 Bioimager System using a 20x objective and merged using the BD AttoVison ™ software.  This antibody also stained SH-SY5Y, SK-N-SH, U87 and U373 cells using both the Triton X100 and methanol fix/perm protocols (see Recommended Assay Procedure; Bioimaging protocol link).

Product Details
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BD Transduction Laboratories™
Rat (QC Testing)
Mouse IgG2b, κ
Human Tau Recombinant Protein
Western blot (Routinely Tested), Bioimaging, Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
50-68 kDa
250 µg/ml
AB_397999
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. Triton is a trademark of the Dow Chemical Company.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610672 Rev. 2
Antibody Details
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15/Tau

In addition to tubulin, microtubules have other components named Microtubule-associated proteins (MAPs), which include the high molecular MAPs (200-400 kDa) and the Tau proteins (55-70 kDa). These proteins co-purify with the microtubules during repeated cycles of microtubule assembly and disassembly. Both MAPs and Tau proteins extend from the side of the microtubules. They form cross-bridges in neurons and bind tubulin by their C-terminal region. The heterogeneity in the molecular weight of Tau proteins is due to post-translational modifications and different numbers of tandem repeats in their C-terminal domain. Tau isoforms are selectively expressed in unique subsets of neurons and regulated during development. The highly phosphorylated Tau proteins have also been detected in the neurofibrillary tangles of Alzheimer's patients.

610672 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610672 Rev.2
Citations & References
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Development References (5)

  1. Alonso AC, Grundke-Iqbal I, Iqbal K. Alzheimer's disease hyperphosphorylated tau sequesters normal tau into tangles of filaments and disassembles microtubules. Nat Med. 1996; 2(7):783-787. (Biology). View Reference
  2. Goedert M, Spillantini MG, Potier MC, Ulrich J, Crowther RA. Cloning and sequencing of the cDNA encoding an isoform of microtubule-associated protein tau containing four tandem repeats: differential expression of tau protein mRNAs in human brain. EMBO J. 1989; 8(2):393-399. (Biology). View Reference
  3. Kanai Y, Hirokawa N. Sorting mechanisms of tau and MAP2 in neurons: suppressed axonal transit of MAP2 and locally regulated microtubule binding. Neuron. 1995; 14(2):421-432. (Biology). View Reference
  4. Takeda K, Hatai T, Hamazaki TS, Nishitoh H, Saitoh M, Ichijo H. Apoptosis signal-regulating kinase 1 (ASK1) induces neuronal differentiation and survival of PC12 cells. J Biol Chem. 2000; 275(13):9805-9813. (Biology: Western blot). View Reference
  5. Wilson DM, Binder LI. Free fatty acids stimulate the polymerization of tau and amyloid beta peptides. In vitro evidence for a common effector of pathogenesis in Alzheimer's disease. Am J Pathol. 1997; 150(6):2181-2195. (Biology). View Reference
View All (5) View Less
610672 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.