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Purified Mouse Anti-Mouse iNOS
Purified Mouse Anti-Mouse iNOS

Western blot analysis of iNOS/NOS Type II on a cell lysate from mouse macrophages (RAW 264.7) stimulated with 10 ng/mL IFNγ and 1 µg/mL LPS for 12 hours. Lane 1: 1:250, Lane 2: 1:500, Lane 3: 1:1000 dilution of the mouse anti- mouse iNOS/NOS Type II antibody.

Western blot analysis of iNOS/NOS Type II on a cell lysate from mouse macrophages (RAW 264.7) stimulated with 10 ng/mL IFNγ and 1 µg/mL LPS for 12 hours. Lane 1: 1:250, Lane 2: 1:500, Lane 3: 1:1000 dilution of the mouse anti- mouse iNOS/NOS Type II antibody.

Product Details
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BD Transduction Laboratories™
NOS Type II
Mouse (QC Testing)
Mouse IgG1
Mouse iNOS aa. 772-787
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
130 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610599 Rev. 2
Antibody Details
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2/iNOS

Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In macrophages and other cell types, NOS (iNOS or macNOS) activity increases following exposure to cytokines (IFN-γ, TNF-α, and IL-1) and microbial products (lipopolysaccharide (LPS)). iNOS isactivated independently of Ca2+/calmodulin and its level of expression is tightly controlled by several transcription factors, including NFκB. Data indicates that TGF-ß affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue, iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain.

610599 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610599 Rev.2
Citations & References
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Development References (4)

  1. Koprowski H, Zheng YM, Heber-Katz E, et al. In vivo expression of inducible nitric oxide synthase in experimentally induced neurologic diseases. Proc Natl Acad Sci U S A. 1993; 90(7):3024-3027. (Biology). View Reference
  2. Nathan C, Xie QW. Regulation of biosynthesis of nitric oxide. J Biol Chem. 1994; 269(19):13725-13728. (Biology). View Reference
  3. Vodovotz Y, Bogdan C, Paik J, Xie QW, Nathan C. Mechanisms of suppression of macrophage nitric oxide release by transforming growth factor beta. J Exp Med. 1993; 178(2):605-613. (Biology). View Reference
  4. Xie QW, Cho HJ, Calaycay J, et al. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science. 1992; 256(5054):225-228. (Biology). View Reference
View All (4) View Less
610599 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.