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      Purified Mouse Anti-Kalinin B1
      Product Details
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      BD Transduction Laboratories™
      Human (QC Testing), Dog (Tested in Development)
      Mouse IgG1
      Human Kalinin B1 aa. 1009-1170
      Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
      140 kDa
      250 µg/ml
      AB_397802
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      610423 Rev. 1
      Antibody Details
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      17/Kalinin B1

      Kalinin is an epithelial cell laminin. It is a structural component of the dermal-epidermal junction of skin and basement membrane. The full molecule is composed of a 200 kDa A subunit, a 155 kDa B2 subunit, and a 140 kDa B1 subunit. Another B1k subunit with a predicted molecular weight of 126.9 kDa is encoded by a 3.9kb gene. The apparent molecular weight of Kalinin B1k is 140 kDa due to glycosylation. Its structure is divided into a long arm and a short arm domain that bind and crosslink with the other kalinin subunits.

      610423 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610423 Rev.1
      Citations & References
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      Development References (3)

      1. Gerecke DR, Wagman DW, Champliaud MF, Burgeson RE. The complete primary structure for a novel laminin chain, the laminin B1k chain. J Biol Chem. 1994; 269(15):11073-11080. (Biology). View Reference
      2. Hirosaki T, Mizushima H, Tsubota Y, Moriyama K, Miyazaki K. Structural requirement of carboxyl-terminal globular domains of laminin alpha 3 chain for promotion of rapid cell adhesion and migration by laminin-5. J Biol Chem. 2000; 275(29):22495-22502. (Clone-specific: Western blot). View Reference
      3. Hirosaki T, Tsubota Y, Kariya Y, Moriyama K, Mizushima H, Miyazaki K. Laminin-6 is activated by proteolytic processing and regulates cellular adhesion and migration differently from laminin-5. J Biol Chem. 2002; 277(51):49287-49295. (Clone-specific: Western blot). View Reference
      610423 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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