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Purified Mouse Anti-Human SLP-76
Purified Mouse Anti-Human SLP-76

Western blot analysis of SLP-76 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human SLP-76 antibody.

Purified Mouse Anti-Human SLP-76

Immunofluorescence staining of Jurkat cells (Human T-cell leukemia; ATCC TIB-152).

Western blot analysis of SLP-76 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human SLP-76 antibody.

Immunofluorescence staining of Jurkat cells (Human T-cell leukemia; ATCC TIB-152).

Product Details
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BD Transduction Laboratories™
SH2 domain-containing Leukocyte Protein
Human (QC Testing)
Mouse IgG1
Human SLP-76 aa. 369-520
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
76 kDa
250 µg/ml
AB_398059
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610736 Rev. 1
Antibody Details
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8/SLP-76

SLP-76 (SH2 domain-containing Leukocyte Protein) is a tyrosine phosphoprotein that is involved in the TCR mediated intracellular signaling pathway. It may be involved in the signaling pathways of peripheral blood leukocytes, thymic/splenic cells, and in human T, B, and monocytic cell lines. SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling pathways, such as the SH2 domain in the C-terminus, the amino-terminus three 17-amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P), and a proline rich region. SLP-76 has been shown to associate with Grb2, PLC-γ, SLAP-130, and Vav-all of which are part of the signaling cascade in T cells. ZAP-70 appears to phosphorylate SLP-76, allowing phosphorylated Vav to bind and, together the SLP-76/Vav complex, cooperatively stimulates NF-AT and IL-2 gene activation. Overexpression of SLP-76 has been reported to increase the TCR-mediated transcription of the IL-2 gene. Furthermore, SHP-1 associates with SLP-76 in normal splenic B cells which suggests a role for SLP-76 in BCR signaling.

610736 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610736 Rev.1
Citations & References
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Development References (5)

  1. Harder T, Kuhn M. Selective accumulation of raft-associated membrane protein LAT in T cell receptor signaling assemblies. J Cell Biol. 2000; 151(2):199-208. (Biology: Western blot). View Reference
  2. Jackman JK, Motto DG, Sun Q. Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells. J Biol Chem. 1995; 270(13):7029-7032. (Biology). View Reference
  3. Tuosto L, Michel F, Acuto O. p95vav associates with tyrosine-phosphorylated SLP-76 in antigen-stimulated T cells. J Exp Med. 1996; 184(3):1161-1166. (Biology). View Reference
  4. Wu J, Motto DG, Koretzky GA, Weiss A. Vav and SLP-76 interact and functionally cooperate in IL-2 gene activation. Immunity. 1996; 4(6):593-602. (Biology). View Reference
  5. Zhang W, Trible RP, Zhu M. Association of Grb2, Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues. Effect of LAT tyrosine mutations on T cell angigen receptor-mediated signaling. J Biol Chem. 2000; 275(30):23355-23361. (Biology: Western blot). View Reference
View All (5) View Less
610736 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.