Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
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TNFα (Tumor Necrosis Factor α) is a pleiotropic cytokine that conveys either beneficial (prevention of cancers and infection, activation of inflammatory responses) or detrimental (mediation of septic shock, inflammation, and apoptosis) effects to the host. It is produced and secreted primarily by monocytes and macrophages in response to stimulation with lipopolysaccharide (LPS), a principle endotoxic component. LPS-induced TNFα factor (LITAF) is a nuclear factor that was purified as a result of its ability to bind to a DNA fragment from the TNFα promoter region. LITAF expression is dependent on LPS induction and PMA differentiation in THP-1 cells and is observed at high levels in spleen, lymph node, and peripheral blood leukocytes. Antisense inhibition of LITAF causes a reduction in TNFα expression in THP-1 cells. In addition, p53 induces the expression of a transcript called PIG7, which has 98% homology with LITAF. Thus, LITAF is thought to link p53 pathways with the induction of TNFα gene expression in response to LPS stimulation and to function, possibly as a transcription factor, to regulate the expression of this highly potent cytokine. This antibody was generated to the LITAF aa 1-212 sequence (Genbank accession # U77396). Recent reports indicate that the nucleotide coding sequence for LITAF may differ and instead, encode for a protein designated as SIMPLE. Under the sequence for SIMPLE, the LITAF aa 1-212 sequence used to generate this antibody corresponds to the aa 1-126 region of SIMPLE.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (3)
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Moriwaki Y, Begum NA, Kobayashi M, Matsumoto M, Toyoshima K, Seya T. Mycobacterium bovis Bacillus Calmette-Guerin and its cell wall complex induce a novel lysosomal membrane protein, SIMPLE, that bridges the missing link between lipopolysaccharide and p53-inducible gene, LITAF(PIG7), and estrogen-inducible gene, EET-1. J Biol Chem. 2001; 276(25):23065-23076. (Immunogen). View Reference
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Myokai F, Takashiba S, Lebo R, Amar S. A novel lipopolysaccharide-induced transcription factor regulating tumor necrosis factor alpha gene expression: molecular cloning, sequencing, characterization, and chromosomal assignment. Proc Natl Acad Sci U S A. 1999; 96(8):4518-4523. (Biology). View Reference
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Saifi GM, Szigeti K, Wiszniewski W, et al. SIMPLE mutations in Charcot-Marie-Tooth disease and the potential role of its protein product in protein degradation. Hum Mutat. 2005; 25(4):372-383. (Immunogen). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
