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Purified Mouse Anti-AKAP95
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1, κ
Human AKAP95 aa. 460-781
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
95 kDa
250 µg/ml
AB_398307
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610994 Rev. 2
Antibody Details
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47/AKAP95

Induction of cAMP results in the activation of the cAMP-dependent protein kinase (PKA) which, in turn, phosphorylates and regulates downstream effectors. The localization and, hence, the specificity of PKA action is mediated by the interaction of four different PKA regulatory subunits (RIα, RIβ, RIIα, and RIIβ) and specific PKA anchoring proteins. Several proteins have been identified as PKA type I/II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). Several AKAPs (AKAP79/75, AKAP350, and AKAP250) associate with PKA type II and direct its localization to cortical actin, centrosomes, and filopodia. AKAP95 is a specific nuclear anchoring protein that contains two zinc finger motifs and an RII binding domain. Although AKAP95 does not interact with RIIβ, it has a high affinity for RIIα. During interphase, AKAP95 is bound to DNA. However, entry into mitosis results in AKAP95 detachment from the DNA and association with RIIα. Although the specific function of this AKAP95/RIIα heterodimer is unknown, its formation is cell cycle-dependent and may be essential for proper progression through the cell cycle.

610994 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610994 Rev.2
Citations & References
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Development References (5)

  1. Collas P, Le Guellec K, Taskén K. The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in chromatin condensation at mitosis. J Cell Biol. 1999; 147(6):1167-1180. (Clone-specific: Functional assay). View Reference
  2. Eide T, Coghlan V, Orstavik S. Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. Exp Cell Res. 1998; 238(2):305-316. (Biology). View Reference
  3. Schillace RV, Andrews SF, Liberty GA, Davey MP, Carr DW. Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes. J Immunol. 2002; 168(4):1590-1599. (Clone-specific: Immunofluorescence, Western blot). View Reference
  4. Steen RL, Cubizolles F, Le Guellec K, Collas P. A kinase-anchoring protein (AKAP)95 recruits human chromosome-associated protein (hCAP)-D2/Eg7 for chromosome condensation in mitotic extract. J Cell Biol. 2000; 149(3):531-536. (Clone-specific: Western blot). View Reference
  5. Steen RL, Martins SB, Taskén K, Collas P. Recruitment of protein phosphatase 1 to the nuclear envelope by A-kinase anchoring protein AKAP149 is a prerequisite for nuclear lamina assembly. J Cell Biol. 2000; 150(6):1251-1262. (Clone-specific: Western blot). View Reference
View All (5) View Less
610994 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.