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Western blot analysis of AKAP79 on SW13 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-AKAP79.
Immunofluorescent staining of endothelial cells.
BD Transduction Laboratories™ Purified Mouse Anti-AKAP79
BD Transduction Laboratories™ Purified Mouse Anti-AKAP79
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
The type II cAMP-dependent Protein Kinase (PKA) is compartmentalized within the cell. To maintain this localization of type II PKAs, the regulatory subunit (RII) interacts with specific RII-anchoring proteins. For instance, attachment of type II PKA to the cytoskeleton occurs through the binding of RII to microtubule-associated protein 2 (MAP2). In brain, several proteins have been identified as PKA type II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP79 is a 79kDa human RII-anchoring protein. AKAP, PKA type II, and calcineurin (PP2B) can form a tertiary complex, suggesting that both PKA and calcineurin are targeted by a common protein to subcellular sites where they regulate the phosphorylation status of key substrates.
Development References (4)
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Carr DW, Stofko-Hahn RE, Fraser ID, Cone RD, Scott JD. Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79.. J Biol Chem. 1992; 267(24):16816-23. (Biology). View Reference
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Coghlan VM, Perrino BA, Howard M. Association of protein kinase A and protein phosphatase 2B with a common anchoring protein. Science. 1995; 267(5194):108-111. (Biology). View Reference
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Jicha GA, Weaver C, Lane E. cAMP-dependent protein kinase phosphorylations on tau in Alzheimer's disease. J Neurosci. 1999; 19(17):7486-7494. (Clone-specific: Immunohistochemistry, Western blot). View Reference
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Schillace RV, Andrews SF, Liberty GA, Davey MP, Carr DW. Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes. J Immunol. 2002; 168(4):1590-1599. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
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