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Purified Mouse Anti-Acetylcholine Receptor α
Product Details
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BD Transduction Laboratories™
Mouse (QC Testing), Rat (Tested in Development)
Mouse IgG2a
Rat Acetylcholine Receptor α aa. 332-457
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
49 kDa
250 µg/ml
AB_398302
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610989 Rev. 1
Antibody Details
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26/Acetylcholine Receptor α

Acetylcholine is an amine neurotransmitter at the neuromuscular junction. It is released from the presynaptic membrane of a cholinergic synapse into the synaptic cleft. It diffuses across the cleft and binds acetylcholine receptors (AChR) on the postsynaptic membrane. Receptor binding induces postsynaptic membrane depolarization and the generation of an action potential that produces effects such as muscle contraction. The AChR is a 250kDa pentameric complex of four transmembrane subunits in a stoichiometry of α2βγδ. In response to ligand binding, all subunits participate in the formation of an integral cation channel. However, the acetylcholine binding site is primarily within the α subunit. Myasthenia gravis (MG) is an autoimmune condition in which AchR levels are decreased. Autoantibodies bind and crosslink the AchRs leading to their internalization and degradation. This results in a decreased number of functional AChRs. Patients develop muscular weakness and some voluntary muscle fatigue. However, development of MG is also affected by genetic factors. One of the allelic forms of the AChRα gene appears to significantly contribute to MG susceptibility.

610989 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610989 Rev.1
Citations & References
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Development References (5)

  1. Garchon HJ, Djabiri F, Viard JP, Gajdos P, Bach JF. Involvement of human muscle acetylcholine receptor alpha-subunit gene (CHRNA) in susceptibility to myasthenia gravis. Proc Natl Acad Sci U S A. 1994; 91(11):4668-4672. (Biology). View Reference
  2. Kincer JF, Uittenbogaard A, Dressman J, et al. Hypercholesterolemia promotes a CD36-dependent and endothelial nitric-oxide synthase-mediated vascular dysfunction. J Biol Chem. 2002; 277(26):23525-23533. (Clone-specific: Western blot). View Reference
  3. Schroder B, Reinhardt-Maelicke S, Schrattenholz A. Monoclonal antibodies FK1 and WF6 define two neighboring ligand binding sites on Torpedo acetylcholine receptor alpha-polypeptide. J Biol Chem. 1994; 269(14):10407-10416. (Biology). View Reference
  4. Spencer MJ, Guyon JR, Sorimachi H. Stable expression of calpain 3 from a muscle transgene in vivo: immature muscle in transgenic mice suggests a role for calpain 3 in muscle maturation. Proc Natl Acad Sci U S A. 2002; 99(13):8874-8879. (Clone-specific: Immunohistochemistry, Western blot). View Reference
  5. Tsim KW, Greenberg I, Rimer M, Randall WR, Salpeter MM. Transcripts for the acetylcholine receptor and acetylcholine esterase show distribution differences in cultured chick muscle cells. J Cell Biol. 1992; 118(5):1201-1212. (Biology). View Reference
View All (5) View Less
610989 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.