Purified NA/LE Mouse IgG1 κ Isotype Control

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BD Pharmingen™ Purified NA/LE Mouse IgG1 κ Isotype Control

Name: Purified NA/LE Mouse IgG1 κ Isotype Control
Brand: BD Pharmingen™
SKU: 554721

Clone 107.3

(RUO)

Product Details

Brand: BD Pharmingen™

Isotype: Mouse BALB/c IgG1, κ

Immunogen: TNP-keyhole limpet hemocyanin

Application: ELISA (Routinely Tested), Bioassay (Tested During Development), Intracellular staining (flow cytometry) (Not Recommended)

Concentration: 1.0 mg/ml

RRID: AB_395530

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Purified NA/LE Mouse IgG1 κ Isotype Control RUO

Size 0.5 mg Cat No. 553447

554721 Rev. 6

Antibody Details

107.3

The 107.3 clone has an unknown specificity. Trinitrophenal (TNP), the immunogen, is a hapten not expressed on human or mouse cells. In the absence of specific binding, this antibody may bind non-specifically to Fc receptors. The immunoglobulin from clone 107.3 was selected as an isotype control following screening for low background on a variety of mouse and human tissues.

This material is suitable as an isotype control for matching cytokine-neutralizing antibodies used in bioassay or as a negative control for in vitro and in vivo functional studies.

554721 Rev. 6

Format Details

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

Format: NA/LE

554721 Rev.6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.