Preparation And Storage
Recommended Assay Procedures
Applications include immunoprecipitation (1-2 µg/ml). Other applications not routinely tested at BD Biosciences Pharmingen include in vitro kinase assays. Raji human Burkitt's lymphoma cells (ATCC CCL-86) are suggested as a positive control.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small proteins that bind to cyclins, cdks, or cyclin-cdk complexes, e.g., p21 and p27[Kip1]. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. Cyclin E is expressed in G1 and associates with cdk2 to form an active kinase. Cyclin E-cdk2 complexes play an important role in regulation of the G1/S restriction checkpoint in the cell cycle. Aberrant expression of cyclin E is associated with oncogenic transformation of cells. Cyclin E migrates at a reduced molecular weight of ~50 kDa.
Clone HE67 recognizes human cyclin E. It does not cross-react with mouse cyclin E. Recombinant human cyclin E was used as immunogen.
Development References (4)
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Faha B, Harlow E, Lees E. The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2. J Virol. 1993; 67(5):2456-2465. (Biology). View Reference
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Keyomarsi K, Herliczek TW.. The role of cyclin E in cell proliferation, development and cancer.. Prog Cell Cycle Res. 1997; 3:171-191. (Biology). View Reference
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Keyomarsi K, O'Leary N, Molnar G, Lees E, Fingert HJ, Pardee AB. Cyclin E, a potential prognostic marker for breast cancer. Cancer Res. 1994; 54(2):380-385. (Biology). View Reference
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Sherr CJ. Mammalian G1 cyclins. Cell. 1993; 73(6):1059-1065. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
