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V450 Rat Anti-Mouse CD146
V450 Rat Anti-Mouse CD146

Multicolor flow cytometric analysis of CD146 expression on C57BL/6 splenocytes. Spleen cells were stained with FITC Rat anti-Mouse CD49b antibody (Cat. No. 553857/561067) and either BD Horizon™ V450 Rat IgG2a, κ Isotype Control (Cat. No. 560377; Left Panel) or BD Horizon™ V450 Rat anti-Mouse CD146 antibody (Cat. No. 562232; Right Panel). Two-color flow cytometric dot plots showing the correlated expression patterns of CD49b versus CD146 (or Ig isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Multicolor flow cytometric analysis of CD146 expression on C57BL/6 splenocytes. Spleen cells were stained with FITC Rat anti-Mouse CD49b antibody (Cat. No. 553857/561067) and either BD Horizon™ V450 Rat IgG2a, κ Isotype Control (Cat. No. 560377; Left Panel) or BD Horizon™ V450 Rat anti-Mouse CD146 antibody (Cat. No. 562232; Right Panel). Two-color flow cytometric dot plots showing the correlated expression patterns of CD49b versus CD146 (or Ig isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Product Details
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BD Horizon™
S-endo 1; S-endo; Muc18; Mcam; Gicerin
Mouse (QC Testing)
Rat IgG2a, κ
CBA/J Mouse lymph node Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
84004
AB_11152959
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
  5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  6. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  7. An isotype control should be used at the same concentration as the antibody of interest.
562232 Rev. 1
Antibody Details
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ME-9F1

The ME-9F1 monoclonal antibody specifically binds to mouse CD146. The CD146 adhesion molecule is a type 1 transmembrane glycoprotein and member of the immunoglobulin superfamily. CD146 is expressed by blood vessel endothelial cells and may play roles in forming intercellular junctions between endothelial cells and influencing the transendothelial migration of other cell types. CD146 is also expressed by some melanoma cell lines, NK cells and neutrophils. CD146 is not detectable on mouse monocytes, dendritic cells, T cells, NKT cells, B cells and smooth muscle cells. Increased expression of CD146 is reportedly associated with NK cell maturation and may be used to characterize different functional NK cell subsets. Activated CD146-positive mouse NK cells reportedly are less cytotoxic and secrete less IFN-γ than their CD146-negative counterparts.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

562232 Rev. 1
Format Details
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V450
BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
V450
Violet 405 nm
405 nm
450 nm
562232 Rev.1
Citations & References
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Development References (4)

  1. Alais S, Allioli N, Pujades C, et al. HEMCAM/CD146 downregulates cell surface expression of beta1 integrins. J Cell Sci. 2001; 114(Pt 10):1847-1859. (Biology). View Reference
  2. Despoix N, Walzer T, Jouve N, et al. Mouse CD146/MCAM is a marker of natural killer cell maturation. Eur J Immunol. 2008; 38(10):2855-2864. (Biology). View Reference
  3. Schrage A, Loddenkemper C, Erben U, et al. Murine CD146 is widely expressed on endothelial cells and is recognized by the monoclonal antibody ME-9F1. Histochem Cell Biol. 2008; 129(4):441-451. (Clone-specific: Flow cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation). View Reference
  4. Yang H, Wang S, Liu Z, et al. Isolation and characterization of mouse MUC18 cDNA gene, and correlation of MUC18 expression in mouse melanoma cell lines with metastatic ability. Gene. 2001; 265(1-2):133-145. (Biology). View Reference
View All (4) View Less
562232 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.