Skip to main content Skip to navigation
R718 Mouse Anti-TCF-7/TCF-1
R718 Mouse Anti-TCF-7/TCF-1

Multicolor flow cytometric analysis of TCF-7/TCF-1 expression by human lymphocytes or mouse splenocytes. Bivariate pseudocolor density plots showing TCF-7/TCF-1 expression (or Ig Isotype control staining) versus CD4 were derived from gated events with the side and forward light-scattering characteristics of intact human lymphocytes (upper plots) or mouse splenocytes (lower plots). Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.  

Upper Plots: Human peripheral blood mononuclear cells (PBMC) were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with PE Mouse Anti-Human CD4 antibody (Cat. No. 555347/561844/561843) and either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; Left Plot) or BD Horizon™ R718 Mouse Anti-TCF-7/TCF-1 antibody (Cat. No. 567587; Right Plot) at 0.5 μg/test.

Lower Plots - Mouse splenic leucocytes were similarly fixed and permeabilized. The cells were then stained with BD Horizon™ BUV395 Rat Anti-Mouse CD4 antibody (Cat. No. 740208) and either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Left Plot) or BD Horizon™ R718 Mouse Anti-TCF-7/TCF-1 antibody (Right Plot) at 0.5 μg/test.

Multicolor flow cytometric analysis of TCF-7/TCF-1 expression by human lymphocytes or mouse splenocytes. Bivariate pseudocolor density plots showing TCF-7/TCF-1 expression (or Ig Isotype control staining) versus CD4 were derived from gated events with the side and forward light-scattering characteristics of intact human lymphocytes (upper plots) or mouse splenocytes (lower plots). Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.  

Upper Plots: Human peripheral blood mononuclear cells (PBMC) were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with PE Mouse Anti-Human CD4 antibody (Cat. No. 555347/561844/561843) and either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; Left Plot) or BD Horizon™ R718 Mouse Anti-TCF-7/TCF-1 antibody (Cat. No. 567587; Right Plot) at 0.5 μg/test.

Lower Plots - Mouse splenic leucocytes were similarly fixed and permeabilized. The cells were then stained with BD Horizon™ BUV395 Rat Anti-Mouse CD4 antibody (Cat. No. 740208) and either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Left Plot) or BD Horizon™ R718 Mouse Anti-TCF-7/TCF-1 antibody (Right Plot) at 0.5 μg/test.

Product Details
Down Arrow Up Arrow


BD Horizon™
TCF7; Transcription factor 7; TCF-1; TCF1; T-cell factor 1
Mouse (QC Testing)
Mouse IgG1, κ
Mouse TCF-7 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Alexa Fluor™ is a trademark of Life Technologies Corporation.
567587 Rev. 1
Antibody Details
Down Arrow Up Arrow
S33-966

The S33-966 monoclonal antibody specifically binds to TCF-7 (Transcription factor 7) which is also known as TCF-1 (T-cell factor 1). TCF-7 is a member of the High Mobility Group (HMG) DNA binding protein family of transcription factors. TCF-7 is expressed in thymocytes, T lymphocytes and proliferating epithelial cells. TCF-7 is expressed in the earliest T cell precursors and serves as a critical transcriptional regulator that is involved in the differentiation of thymocytes to become mature T lymphocytes. TCF-7 is necessary for the survival of immature CD4+CD8+ thymocytes. TCF-7 can also promote the differentiation of mature peripheral T cells to become Th2-like cells. TCF-7 may also function as a transcriptional repressor of certain target genes including CTNNB1 and TCF7L2. The S33-966 antibody crossreacts with human TCF-7.

The antibody was conjugated to BD Horizon™ Red 718, which has been developed exclusively by for BD Biosciences as a better alternative to Alexa Fluor™ 700. BD Horizon™ Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor™ 700.

567587 Rev. 1
Format Details
Down Arrow Up Arrow
R718
The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
R718
Red 627-640 nm
695 nm
718 nm
567587 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Chen Z, Ji Z, Ngiow SF, et al. TCF-1-Centered Transcriptional Network Drives an Effector versus Exhausted CD8 T Cell-Fate Decision. Immunity. 2019; 51(5):840-855. (Clone-specific: Flow cytometry). View Reference
  2. Germar K, Dose M, Konstantinou T, et al. T-cell factor 1 is a gatekeeper for T-cell specification in response to Notch signaling. Proc Natl Acad Sci U S A. 2011; 108(50):20060-20065. (Biology). View Reference
  3. Johnson JL, Georgakilas G, Petrovic J, et al. Lineage-Determining Transcription Factor TCF-1 Initiates the Epigenetic Identity of T Cells.. Immunity. 2018; 48(2):243-257.e10. (Clone-specific: Flow cytometry). View Reference
  4. Weber BN, Chi AW, Chavez A, et al. A critical role for TCF-1 in T-lineage specification and differentiation. Nature. 2011; 476(7358):63-68. (Biology). View Reference
  5. Yu Q, Sharma A, Oh SY, et al. T cell factor 1 initiates the T helper type 2 fate by inducing the transcription factor GATA-3 and repressing interferon-γ. Nat Immunol. 2009; 10(9):992-999. (Biology). View Reference
View All (5) View Less
567587 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.