The TX45 monoclonal antibody recognizes human CD300c which is also known as Leukocyte immunoglobulin-like receptor (LIR), CMRF35-like molecule 6 (CLM-6), or Immunoglobulin superfamily member 16 (IGSF16). CD300c is a type I transmembrane glycoprotein that belongs to the CMRF family within the Ig superfamily. CD300c is primarily expressed on myeloid cells and has also been detected on some T cells, B cells, and NK cells. Amongst freshly-isolated leucocytes, the TX45 antibody only detects CD300c expressed on monocytes. CD300c is involved in the regulation of proinflammatory cytokine production by leucocytes.
The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.